Isolation and Characterization of Microsatellite Loci in the Tree Fern Alsophila spinulosa

Alsophila spinulosa is a famous relict tree fern. Three polymorphic microsatellite makers were developed and characterized using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. The polymorphism was quantified for a single population from Guizhou province, China. The num...

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Bibliographic Details
Published in:American fern journal 2008-01, Vol.98 (1), p.42-45
Main Authors: Zhou, Yuan, Chen, Guo-Pei, Wang, Ting
Format: Article
Language:English
Subjects:
Alsophila
Alsophila spinulosa
Cyatheaceae
DNA
Endangered species
Ferns
Genetic loci
Genetic variation
microsatellite
Microsatellite repeats
Microsatellites
Population genetics
Tree ferns
Tropical regions
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Summary:Alsophila spinulosa is a famous relict tree fern. Three polymorphic microsatellite makers were developed and characterized using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. The polymorphism was quantified for a single population from Guizhou province, China. The number of alleles per locus varied from 2 to 4. The ranges of observed and expected heterozygosity were 0.000–0.750 and 0.218–0.651, respectively. These were the first microsatellites reported for the Cyatheaceae and will be useful for the ongoing population and conservation genetic studies of other remaining extant populations.
ISSN:0002-8444
1938-422X
DOI:10.1640/0002-8444(2008)98[42:IACOML]2.0.CO;2