Testing an association between TLR4 and CXCR1 gene polymorphisms with susceptibility to urinary tract infection in type 2 diabetes in north Indian population

• Published in: Gene 2018-01, Vol.641, p.196-202
• Main Authors: Gond, Dinesh Prasad, Singh, Shivendra, Agrawal, N.K.
• Format: Article
• Language: English
• Subjects: Adult; Alleles; Case-Control Studies; CXCR1 polymorphism; Diabetes Mellitus, Type 2 - genetics; Female; Genetic Predisposition to Disease - genetics; Genotype; Humans; India; Male; Polymorphism, Single Nucleotide - genetics; Receptors, Interleukin-8A - genetics; Toll like receptor 4 polymorphism; Toll-Like Receptor 4 - genetics; Type 2 diabetes mellitus; Urinary tract infection; Urinary Tract Infections - genetics
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2017.10.060

Genetic variations of Toll like receptor 4 (TLR4) and CXC-chemokine receptor type1 (CXCR1), the key elements of innate immune system and their association with urinary tract infection (UTI) were studied in general population. In present study we investigate genetic variation of these genes in diabetic patients (3 to 4 times higher prevalence of UTI in comparison to general population). A total 1100 subjects (318 diabetic patients with UTI, 324 diabetic patients without UTI, 200 non-diabetic UTI patients and 260 age matched healthy control) were enrolled in the study. SNPs of TLR4 rs4986790, rs4986791 and CXCR1 rs2234671 was assessed by PCR-RFLP and PCR-SSP respectively. Statistical analysis revealed that A/G genotype and G allele of TLR4 rs4986790 are significantly associated with UTI in both diabetics and nondiabetic patients in comparison to healthy control. Similarly CT genotype and T allele of TLR4 rs4986791 are also significantly associated with UTI in both groups. We also found that prevalence of A/G genotype of TLR4 rs4986790 and CT genotype of TLR4 rs4986791 are significantly higher in patients of diabetes with UTI in comparison to diabetic patients without UTI. We did not find any association of CXCR1 rs2234671 polymorphism with UTI by comparing with any group. We found that TLR4 rs4986790 and rs4986791 gene polymorphism is a risk for UTI development in both diabetic and nondiabetic patients in north Indian population. •AG genotype and G allele of TLR4 rs4986790 was significantly associated with UTI diabetics and nondiabetic patients.•Similarly CT genotype and T allele of TLR4 rs4986791 was also significantly associated with UTI in diabetics and nondiabetic patients.•CXCR1 rs2234671 polymorphism was not associated with UTI.•The TLR4 gene polymorphism increases susceptibility for UTI.