Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA super(Met)

Human mitochondrial methionine transfer RNA (hmtRNA super(Met) sub(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f super(5)C sub(34)). The role of this modification in (hmtRNA super(Met) sub(CAU)) for the decoding of AUA, as well as AUG, in both t...

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Bibliographic Details
Published in:Nucleic acids research 2008-11, Vol.36 (20), p.6548-6557
Main Authors: Lusic, Hrvoje, Gustilo, Estella M, Vendeix, Franck AP, Kaiser, Rob, Delaney, Michael O, Graham, William D, Moye, Virginia A, Cantara, William A, Agris, Paul F, Deiters, Alexander
Format: Article
Language:English
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Summary:Human mitochondrial methionine transfer RNA (hmtRNA super(Met) sub(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f super(5)C sub(34)). The role of this modification in (hmtRNA super(Met) sub(CAU)) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkn703