Microsatellite DNA markers for Australian geckos

Nine highly polymorphic microsatellite markers were developed for Gehyra variegata from an enriched library screened for tetranucleotide repeats. An average of 19.44 alleles per locus and an average observed heterozygosity of 0.86 were found in 81 samples from fragmented populations in the Western A...

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Bibliographic Details
Published in:Conservation genetics 2006-10, Vol.7 (5), p.795-798
Main Authors: Hoehn, M, Sarre, S. D
Format: Article
Language:English
Subjects:
alleles
Animal populations
extinction
Gehyra
Gekkonidae
Genetic markers
Genetics
heterozygosity
landscapes
loci
microsatellite repeats
population
Pygopodidae
sampling
Species extinction
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Summary:Nine highly polymorphic microsatellite markers were developed for Gehyra variegata from an enriched library screened for tetranucleotide repeats. An average of 19.44 alleles per locus and an average observed heterozygosity of 0.86 were found in 81 samples from fragmented populations in the Western Australian wheatbelt. Different gecko species exhibit varying rates of extinction in fragmented landscapes and the genetic markers were developed to test whether these difference are due to different rates of dispersal. The loci were tested for their ability to cross amplify in 12 species of Australian Gekkonidae and Pygopodidae, in an attempt to identify species which might be amenable to population genetic analysis using these microsatellite markers.
ISSN:1566-0621
1572-9737
DOI:10.1007/s10592-005-9070-0