Use of Pulsed-Field Gel Electrophoresis of Conserved XbaI Fragments for Identification of Swine Salmonella Serotypes

Swine Salmonella isolates (n = 674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with XbaI. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation o...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2007-02, Vol.45 (2), p.472-476
Main Authors: Gaul, Stephen B, Wedel, Stephanie, Erdman, Matthew M, Harris, D.L, Harris, Isabel Turney, Ferris, Kathleen E, Hoffman, Lorraine
Format: Article
Language:English
Subjects:
Animals
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Clinical Veterinary Microbiology
Cluster Analysis
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field - methods
Fundamental and applied biological sciences. Psychology
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Salmonella
Salmonella - classification
Salmonella - genetics
Salmonella Infections, Animal - microbiology
Serotyping
Swine
Swine Diseases - microbiology
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Summary:Swine Salmonella isolates (n = 674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with XbaI. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using XbaI restriction provided a possible alternative method for screening and identifying swine Salmonella serotypes.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.00962-06