Genetic Diversity within and Between Populations of Lathyrus genus (Fabaceae) Revealed by ISSR Markers

In order to evaluate the genetic diversity in Lathyrus genus, the Inter Simple Sequence Repeats method (ISSR) was exploited in five populations. These consisted of two cultivated species belonging to section Lathyrus (L. sativus L. and L. cicera L.) and a wild one belonging to the section Clymenum (...

Full description

Saved in:
Bibliographic Details
Published in:Genetic resources and crop evolution 2006-11, Vol.53 (7), p.1413-1418
Main Authors: Belaïd, Y, Chtourou-Ghorbel, N, Marrakchi, M, Trifi-Farah, N
Format: Article
Language:English
Subjects:
Biological evolution
Deoxyribonucleic acid
DNA
evolution
Fabaceae
field crops
forage crops
forage legumes
Genetic diversity
genetic markers
genetic polymorphism
genetic variation
Lathyrus
Lathyrus cicera
Lathyrus ochrus
Lathyrus sativus
legumes
microsatellite repeats
molecular sequence data
molecular systematics
plant taxonomy
Polymorphism
population genetics
Populations
Primers
Simple sequence repeats
spatial variation
Species
wild plants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to evaluate the genetic diversity in Lathyrus genus, the Inter Simple Sequence Repeats method (ISSR) was exploited in five populations. These consisted of two cultivated species belonging to section Lathyrus (L. sativus L. and L. cicera L.) and a wild one belonging to the section Clymenum (L. ochrus DC.). Two 3'anchored ISSR primers and two unanchored ones, generated a total of 60 useful polymorphic DNA bands. Our data provide evidence of high molecular polymorphism at the intra- and the inter-specific levels showing that both wild and cultivated forms constitute an important pool of diversity. Moreover, among the generated DNA bands, a 500 bp band, totally absent in the banding patterns of the section Clymenum, appears to be a molecular marker of section Lathyrus. Results provided for lineage and suggest recent origin of these species that might have evolved from a common ancestor producing both L. ochrus species and the two other species L. sativus and L. cicera. These relationships support previous studies based on morphological variation and molecular analysis.
ISSN:0925-9864
1573-5109
DOI:10.1007/s10722-005-5680-0