Aloperine induces apoptosis and inhibits invasion in MG-63 and U2OS human osteosarcoma cells

Aloperine (ALO) is a novel type of alkaloid drug that is extracted from S. alopecuroide, and exert an anti-inflammatory, anti-allergenic, antitumor and antiviral effects. In our study, we evaluated the effects and underlying mechanisms of ALO on MG-63 and U2OS osteosarcoma (OS) cells. ALO suppressed...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2018-01, Vol.97, p.45-52
Main Authors: Chen, Shao, Jin, Zhicheng, Dai, Li, Wu, Hongqiang, Wang, Jieke, Wang, Long, Zhou, Zongwei, Yang, Lianghui, Gao, Weiyang
Format: Article
Language:English
Subjects:
Alkaloids - pharmacology
Aloperine
Apoptosis
Apoptosis - drug effects
Apoptosis - physiology
Bone Neoplasms - metabolism
Bone Neoplasms - pathology
Cell Line, Tumor
Cell Survival - drug effects
Cell Survival - physiology
Humans
Invasion
MG-63
Neoplasm Invasiveness - pathology
Neoplasm Invasiveness - prevention & control
Osteosarcoma - metabolism
Osteosarcoma - pathology
Piperidines - pharmacology
Signal Transduction - drug effects
Signal Transduction - physiology
U2OS
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Summary:Aloperine (ALO) is a novel type of alkaloid drug that is extracted from S. alopecuroide, and exert an anti-inflammatory, anti-allergenic, antitumor and antiviral effects. In our study, we evaluated the effects and underlying mechanisms of ALO on MG-63 and U2OS osteosarcoma (OS) cells. ALO suppressed the proliferation and clonogenecity of both cell lines in a dose- and time-dependent manner as observed by CCK-8 and clonogenic survival assays. Data of morphologic changes, DAPI assays and flow cytometry showed that ALO induced apoptosis of OS cells, and the results of western blotting and qRT-PCR indicated that ALO upregulated protein and mRNA of Bax and cleaved caspase-3, while downregulated Bcl-2. Besides, ALO inhibited the invasion of MG-63 and U2OS cells as shown by transwell invasion assay. The protein and mRNA of MMP-2 and MMP-9 were decreased with ALO treatment. ALO also downregulated the protein and mRNA expression of PI3K and p-AKT1. In conclusion, ALO induced apoptosis and inhibited invasion in MG-63 and U2OS cells, which maybe through suppression of PI3K/AKT signaling pathway.
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2017.09.066