Co-production of polyhydroxyalkanoates and carotenoids through bioconversion of glycerol by Paracoccus sp. strain LL1

Polyhydroxyalkanoates (PHA) have emerged as a potential alternative to synthetic plastics, although its large-scale production is limited by the expenditure incurred in feed materials. Co-production of various valuable bioproducts along with PHA has been proposed to alleviate the overall production...

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Bibliographic Details
Published in:International journal of biological macromolecules 2018-02, Vol.107 (Pt B), p.2552-2558
Main Authors: Kumar, Prasun, Jun, Hang-Bae, Kim, Beom Soo
Format: Article
Language:English
Subjects:
Batch Cell Culture Techniques
Carbon - pharmacology
Carotenoids
Carotenoids - metabolism
Cell Proliferation - drug effects
Cell retention culture
Cells, Cultured
Co-production
Glycerol
Glycerol - metabolism
Hydrogen-Ion Concentration
Paracoccus - metabolism
Polyhydroxyalkanoates
Polyhydroxyalkanoates - biosynthesis
Proton Magnetic Resonance Spectroscopy
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Summary:Polyhydroxyalkanoates (PHA) have emerged as a potential alternative to synthetic plastics, although its large-scale production is limited by the expenditure incurred in feed materials. Co-production of various valuable bioproducts along with PHA has been proposed to alleviate the overall production cost. Here, high-yield co-production of PHA and carotenoids was achieved in single step fermentation by Paracoccus sp. LL1. The halophilic bacterial strain could metabolize a wide range of substrates including methanol, lactose, galactose, glycerol, fructose, mannitol, etc. Under batch fermentation using mineral media supplemented with 2% glycerol, Paracoccus sp. LL1 synthesized 3.77gL−1 PHA with concomitant production of 3.6mgL−1 of carotenoids after 96h. An enhancement of 2.2-folds in total dry cell weight (DCW) was achieved through cell retention culture of Paracoccus sp. LL1, resulting in a maximum DCW (24.2gL−1) containing PHA (39.3%, ww−1) with concomitant enhancement in the production of total carotenoids. Paracoccus sp. LL1 could convert glycerol into carotenoids and PHA to a level of 7.14mgL−1 and 9.52gL−1, respectively. It is the first report showing value addition in PHA production process through co-production of high value carotenoids by Paracoccus sp. using glycerol as substrate.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2017.10.147