Use of sodium trimetaphosphate in the inhibition of dentin matrix metalloproteinases and as a remineralizing agent

• Published in: Journal of dentistry 2018-01, Vol.68, p.34-40
• Main Authors: Gonçalves, Rafael Simões, Candia Scaffa, Polliana Mendes, Giacomini, Marina Ciccone, Rabelo Buzalaf, Marília Afonso, Honório, Heitor Marques, Wang, Linda
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Analysis of Variance; Animals; Brazil; Calcium; Calcium Hydroxide - pharmacology; Calcium phosphates; Cattle; Collagen; Demineralization; Demineralizing; Dental caries; Dentin; Dentin - drug effects; Dentistry; Enamel; Enzymes; Fluorides; Hardness; Hardness - drug effects; Humans; Hydrogen-Ion Concentration; In Vitro Techniques; Lesions; Matrix Metalloproteinase 2 - drug effects; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - drug effects; Matrix Metalloproteinase 9 - metabolism; Matrix Metalloproteinase Inhibitors - pharmacology; Matrix metalloproteinases; Matrix Metalloproteinases - drug effects; Matrix Metalloproteinases - metabolism; Mechanical properties; Mineralization; Molecular weight; Phosphorylation; Polyphosphates - pharmacology; Protease inhibitors; Protease Inhibitors - pharmacology; Proteins; Sodium; Sodium Fluoride - pharmacology; Studies; Tooth remineralization; Tooth Remineralization - methods; Young Adult
• ISSN: 0300-5712; 1879-176X
• DOI: 10.1016/j.jdent.2017.10.009

Because of its ability to act as an antiproteolytic agent, the effect of sodium trimetaphosphate (STMP) against specific enzymes extracted from sound dentin and its performance under acidic challenge on demineralized dentin were investigated. The antiproteolytic potential of STMP (0.5%, 1.0%, and 1.5%) was assessed in triplicate by zymography. For the evaluation of remineralization activity, 50 bovine-root dentin specimens were selected and randomly divided into 5 groups (n = 10). Three areas were determined for each specimen: 1) control (no treatment); 2) demineralized (artificial caries-like challenge); 3) treated (demineralized and subjected to pH-cycling for 7 days, and treated for 10 min with 1.5% STMP, 1.5% STMP + calcium hydroxide (Ca[OH]2), 1.5% STMP + sodium fluoride (NaF), NaF, or deionized H2O). The dentin specimens were analyzed for superficial hardness (SH) and cross-sectional hardness (CSH) at different depths (10, 30, 50, 70, 90, 110, and 220 μm) using a Knoop penetrator (10 g/10 s). Statistical analyses were performed with analysis of variance (ANOVA) and Tukey tests (p