Toxic Alexandrium minutum (Dinophyceae) from Vietnam with new gonyautoxin analogue

• Published in: Harmful algae 2007-04, Vol.6 (3), p.321-331
• Main Authors: Lim, Po-Teen, Sato, Shigeru, Van Thuoc, Chu, Tu, Pham The, Huyen, Nguyen Thi Minh, Takata, Yoshinobu, Yoshida, Makoto, Kobiyama, Atsushi, Koike, Kazuhiko, Ogata, Takehiko
• Format: Article
• Language: English
• Subjects: Alexandrium; Alexandrium minutum; Algae; Animal and plant ecology; Animal, plant and microbial ecology; Autoecology; Biological and medical sciences; Dinophyceae; Fundamental and applied biological sciences. Psychology; Large subunit ribosomal RNA; Marine; Morphology paralytic shellfish toxins; Plant cytology, morphology, systematics, chorology and evolution; Plants and fungi; Thallophyta; Vietnam
• ISSN: 1568-9883; 1878-1470
• DOI: 10.1016/j.hal.2006.04.004

Clonal cultures of Alexandrium species collected from a shrimp pond on the northern coast of Vietnam were established and morphologically identified as Alexandrium minutum. Nucleotide sequences of domains 1 and 2 of the large subunit ribosomal (LSU) rRNA gene showed high sequence similarity to A. minutum isolates from Malaysia. Paralytic shellfish toxin profile of the clones was characterized by the dominance of GTX4, GTX1, and NEO. GTX3, GTX2, and dcSTX were also present in trace amount. Toxin content varied among the strains and growth stages, ranged from 3.0 to 12.5 fmol cell −1. In addition to these known toxin components, a new gonyautoxin derivative was detected by HPLC, eluting between GTX4 and GTX1. The peak of this compound disappeared under non-oxidizing HPLC condition but unchanged either after treated with 0.05 M ammonium phosphate/10% mercaptoethanol or 0.1N HCl hydrolysis. LCMS ion scanning showed a parental ion of [ M + H] + at m/ z 396, [ M − SO 3] + at m/ z 316, and [ M − SO 4] + at m/ z 298. Based on these results, the derivative was identified as deoxy-GTX4-12ol, and this represents the first report of this toxin analogue.