A Screen for Schizosaccharomyces pombe Mutants Defective in Rereplication Identifies New Alleles of rad4 super(+), cut9 super(+) and psf2 super(+)

Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cd...

Full description

Saved in:
Bibliographic Details
Published in:Genetics (Austin) 2005-01, Vol.169 (1)
Main Authors: Gomez, Eliana B, Angeles, Vanessa T, sburg, Susan L
Format: Article
Language:English
Subjects:
Schizosaccharomyces pombe
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cdc13). Of the 42 rereplication-defective mutants analyzed, we were able to clone complementing plasmids for 10. This screen identified new alleles of the APC subunit cut9 super(+) the initiation/checkpoint factor rad4 super(+)cut5 super(+) and the first mutant allele of psf2 super(+) a subunit of the novel GINS replication complex. Other genes identified are likely to play general roles in gene expression and protein localization.
ISSN:0016-6731
DOI:10.1534/genetics.104.034231