Determination of homocysteine and other low-molecular-weight amino thiols in blood plasma

A current approach to determining low-molecular-weight amino thiols (cysteine, homocysteine, and glutathione) in model samples and blood plasma is considered. Procedures for determining 2-100 mu M homocysteine in blood plasma with the use of microcolumn chromatography and capillary electrophoresis w...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2006-11, Vol.61 (11), p.1093-1099
Main Authors: Melnikov, I. O., Nazimov, I. V., Stukacheva, E. A., Glubokov, Yu. M.
Format: Article
Language:English
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Summary:A current approach to determining low-molecular-weight amino thiols (cysteine, homocysteine, and glutathione) in model samples and blood plasma is considered. Procedures for determining 2-100 mu M homocysteine in blood plasma with the use of microcolumn chromatography and capillary electrophoresis were developed. Photometric and fluorimetric detection techniques were used to identify amino thiols. Monobromobimane and 5-iodoacetamidofluorescein were used as labels. Mass spectrometry was used to confirm the structures of test amino thiol derivatives.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934806110098