Genetic Relationships of some PCNA Persimmons (Diospyros kaki Thunb.) from China and Japan revealed by SRAP analysis

Sequence-related amplified polymorphism (SRAP) marker was employed to analyze genetic relationships of 10 Chinese, 11 Japanese varieties of persimmon and its 6 relatives. Twenty out of 72 primer combinations could amplify clearly and consistently. Just only one primer set could discriminate all the...

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Bibliographic Details
Published in:Genetic resources and crop evolution 2006-12, Vol.53 (8), p.1597-1603
Main Authors: Guo, Da-Long, Luo, Zheng-Rong
Format: Article
Language:English
Subjects:
Amplification
astringency
Cluster analysis
cultivar identification
cultivars
Diospyros
Diospyros kaki
Gene polymorphism
genetic markers
Genetic relationship
genetic relationships
Genotypes
Mathematical analysis
molecular sequence data
nucleotide sequences
Persimmons
plant breeding
Plant reproduction
Pollination
Polymorphism
Proliferating cell nuclear antigen
provenance
sequence-related amplified polymorphism
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Summary:Sequence-related amplified polymorphism (SRAP) marker was employed to analyze genetic relationships of 10 Chinese, 11 Japanese varieties of persimmon and its 6 relatives. Twenty out of 72 primer combinations could amplify clearly and consistently. Just only one primer set could discriminate all the genotypes used in this study. The genetic relationships were analyzed using unweighted pair-group method of arithmetic average cluster analysis (UPGMA) and principal coordinated analysis (PCOA). The results showed: Chinese varieties were grouped into two clusters, while the Japanese varieties were grouped into one cluster with two subgroups. The persimmon relatives were clustered together. Probably, Chinese and Japanese Pollination-constant and Non-astringent (PCNA) persimmons had different genetic background. 'Eshi No.1' from Hubei province was close to Male type 6. 'Xiangxitianshi' from Xiangxi municipality, Hunan province, had a high similarity coefficient with 'Maekawa-Jirou' and was clustered in the Japanese varieties group, indicating that 'Xiangxitianshi' maybe close to the Japanese variety in terms of genetic background. The Chinese Pollination-constant and Astringent (PCA) and PCNA persimmons were not distinctly distinguished implying that the non-astringent trait of Chinese PCNA type might result from the PCA type.
ISSN:0925-9864
1573-5109
DOI:10.1007/s10722-005-8717-5