Identification of female-specific blood stains using a 17β-estradiol-targeted aptamer-based sensor

Blood stain evidence obtained from a violent crime scene provides decisive clues that can enable a case to be solved through forensic analyses such as genetic identification. However, collected samples usually contain a mixture of biological material from different sources, making genetic identifica...

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Bibliographic Details
Published in:International journal of legal medicine 2018, Vol.132 (1), p.91-98
Main Authors: Kim, Joo-Young, Park, Jung-Hyun, Kim, Man Il, Lee, Hye Hyeon, Kim, Hye Lim, Jeong, Kyu-Sik, Moon, Sang-Ok, Kang, Pil-Won, Park, Ki-Won, Lee, Yang-Han, Chun, Byung-Won
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aptamers, Nucleotide
Biological materials
Biosensing Techniques - methods
Blood
Blood Stains
Child
Crime
Deoxyribonucleic acid
DNA
DNA - isolation & purification
DNA Fingerprinting
Electrophoresis, Capillary
Estradiol - analysis
Estradiol - chemistry
Female
Females
Fluorescence
Forensic Medicine
Forensic Medicine - methods
Genotype
Humans
Identification
Light
Medical Law
Medicine & Public Health
Microsatellite Repeats
Middle Aged
Original Article
Polymerase Chain Reaction
Sensors
Sex hormones
Spectrometry, Fluorescence
Young Adult
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Description
Summary:Blood stain evidence obtained from a violent crime scene provides decisive clues that can enable a case to be solved through forensic analyses such as genetic identification. However, collected samples usually contain a mixture of biological material from different sources, making genetic identification difficult. To address this issue, we developed an activatable aptamer sensor targeting 17β-estradiol for detection of female-specific blood in mixed samples. With the sensor, we were able to detect blood originating from females using a variable light source (495 nm). The sensor was especially sensitive to blood from young females (10–40 years) but not to blood from older females (≥ 50 years). Genomic DNA was extracted from the female blood specimens identified by this method and used for quantification and short tandem repeat genotyping. We confirmed that there was no fluorescence interference from the aptamer sensor. These results indicate that this novel aptamer sensor can be used to analyze evidentiary blood samples and thereby facilitate subsequent genetic identification.
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-017-1718-z