LncRNA CASC2 inhibited the viability and induced the apoptosis of hepatocellular carcinoma cells through regulating miR‐24‐3p

Cancer susceptibility candidate 2 (CASC2), a recently discovered long non‐coding RNA (lncRNA), was confirmed to play numerous roles in several human cancers. However, the involvement and concrete mechanism of CASC2 in hepatocellular carcinoma (HCC) still need to be further elucidated. The relative e...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2018-08, Vol.119 (8), p.6391-6397
Main Authors: Fan, Ji‐Chang, Zeng, Fei, Le, Yi‐Guan, Xin, Lin
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Biotechnology
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - pathology
Female
Gene Expression Regulation, Neoplastic
Growth rate
Hep G2 Cells
Hepatocellular carcinoma
Humans
Inhibition
Liver cancer
Liver Neoplasms - genetics
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
lncRNA CASC2
Male
Mice
Mice, Inbred BALB C
Mice, Nude
MicroRNAs - biosynthesis
MicroRNAs - genetics
miR‐24‐3p
Ribonucleic acid
RNA
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
RNA, Neoplasm - genetics
RNA, Neoplasm - metabolism
Tissues
Tumorigenesis
Tumors
viability
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Summary:Cancer susceptibility candidate 2 (CASC2), a recently discovered long non‐coding RNA (lncRNA), was confirmed to play numerous roles in several human cancers. However, the involvement and concrete mechanism of CASC2 in hepatocellular carcinoma (HCC) still need to be further elucidated. The relative expressions of CASC2 and miR‐24‐3p in HCC tissue and cell lines were determined by quantitative real‐time PCR (qRT‐PCR). The effects of CASC2 and miR‐24‐3p on HCC cells were further assessed via cell viability and apoptosis. In vivo tumorigenesis assay was performed to verify the inhibition effect of CASC2 on the tumor growth and further clarify the important role of miR‐24‐3p in this mechanism. Compared with the paired normal tissues, the relative expression of CASC2 significantly reduced in the HCC tissues, while miR‐24‐3p as determined by qRT‐PCR obviously increased in the HCC tissues. This observation was also found in HCC cell lines. Meanwhile, the expression of CASC2 was negatively related to miR‐24‐3p expression in the HCC tissues (r = −0.804, P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.26479