The 3D characteristics of post‐traumatic syringomyelia in a rat model: a propagation‐based synchrotron radiation microtomography study

• Published in: Journal of synchrotron radiation 2017-11, Vol.24 (6), p.1218-1225
• Main Authors: Liao, Shenghui, Ni, Shuangfei, Cao, Yong, Yin, Xianzhen, Wu, Tianding, Lu, Hongbin, Hu, Jianzhong, Wu, Hao, Lang, Ye
• Format: Article
• Language: English
• Subjects: Animals; Feasibility Studies; Fluorescence; Holes; Image detection; Imaging techniques; Kaolin; Male; Microtomography; Morphology; phase‐contrast tomography; post‐traumatic syringomyelia; Propagation; propagation‐based synchrotron radiation microtomography; Rats; Rats, Sprague-Dawley; Rodents; Spinal cord; Spinal Cord - diagnostic imaging; Spinal Cord - pathology; spinal cord injury; Synchrotron radiation; Syringomyelia - diagnostic imaging; Syringomyelia - pathology; three‐dimensional; Visualization; X-Ray Microtomography - methods
• ISSN: 1600-5775; 0909-0495; 1600-5775
• DOI: 10.1107/S1600577517011201

Many published literature sources have described the histopathological characteristics of post‐traumatic syringomyelia (PTS). However, three‐dimensional (3D) visualization studies of PTS have been limited due to the lack of reliable 3D imaging techniques. In this study, the imaging efficiency of propagation‐based synchrotron radiation microtomography (PB‐SRµCT) was determined to detect the 3D morphology of the cavity and surrounding microvasculature network in a rat model of PTS. The rat model of PTS was established using the infinite horizon impactor to produce spinal cord injury (SCI), followed by a subarachnoid injection of kaolin to produce arachnoiditis. PB‐SRµCT imaging and histological examination, as well as fluorescence staining, were conducted on the animals at the tenth week after SCI. The 3D morphology of the cystic cavity was vividly visualized using PB‐SRµCT imaging. The quantitative parameters analyzed by PB‐SRµCT, including the lesion and spared spinal cord tissue area, the minimum and maximum diameters in the cystic cavity, and cavity volume, were largely consistent with the results of the histological assessment. Moreover, the 3D morphology of the cavity and surrounding angioarchitecture could be simultaneously detected on the PB‐SRµCT images. This study demonstrated that high‐resolution PB‐SRµCT could be used for the 3D visualization of trauma‐induced spinal cord cavities and provides valuable quantitative data for cavity characterization. PB‐SRµCT could be used as a reliable imaging technique and offers a novel platform for tracking cavity formation and morphological changes in an experimental animal model of PTS. The ability of synchrotron radiation phase‐contrast microtomography to visualize the three‐dimensional morphology of the spinal cord cavity induced by trauma is shown. It could be used as a reliable tool for tracking cavity formation and morphological changes in a post‐traumatic syringomyelia animal model.