The proteome of the human neuroblastoma cell line SH-SY5Y: An enlarged proteome

The human neuroblastoma cell line SH-SY5Y (ATCC: CRL-2266) is widely used as a neural cellular model system. The hitherto existing proteome data (115 proteins) are here extended. A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE wi...

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Published in:Biochimica et biophysica acta 2008-07, Vol.1784 (7), p.983-985
Main Authors: Gilany, Kambiz, Van Elzen, Roos, Mous, Kim, Coen, Edmond, Van Dongen, Walter, Vandamme, Stefaan, Gevaert, Kris, Timmerman, Evy, Vandekerckhove, Joël, Dewilde, Sylvia, Van Ostade, Xaveer, Moens, Luc
Format: Article
Language:English
Subjects:
2D-LC-MALDI-TOF/TOF-MS
2D-PAGE
Cell Line, Tumor
Electrophoresis, Polyacrylamide Gel
Humans
N-terminal COFRADIC
Neoplasm Proteins - metabolism
Neuroblastoma - metabolism
Neuroblastoma - pathology
Proteome
Proteome analysis
SDS-PAGE nano-LC–MS/MS
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:The human neuroblastoma cell line SH-SY5Y (ATCC: CRL-2266) is widely used as a neural cellular model system. The hitherto existing proteome data (115 proteins) are here extended. A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LC–MS/MS, N-terminal COFRADIC analysis with nano-LC–MS/MS and 2D-PAGE with MALDI-TOF/TOF-MS peptide mass fingerprinting. The obtained proteome profile of this cell line is discussed.
ISSN:1570-9639
0006-3002
1878-1454
DOI:10.1016/j.bbapap.2008.03.003