Differential display analysis of gene expression in Etrog citron leaves infected by Citrus viroid III

Citrus are natural hosts of several viroids, which are plant pathogens composed exclusively of a non-protein-coding, small single-stranded circular RNA that is able to replicate autonomously in susceptible hosts. They are responsible for symptoms such as stunting, leaf epinasty, and chlorosis. Citru...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2007-04, Vol.1769 (4), p.228-235
Main Authors: Tessitori, Matilde, Maria, Giovanna, Capasso, Clemente, Catara, Giuliana, Rizza, Serena, De Luca, Viviana, Catara, Antonino, Capasso, Antonio, Carginale, Vincenzo
Format: Article
Language:English
Subjects:
Citrus - genetics
Citrus - virology
Citrus medica
Citrus viroids
Dwarfing
Gene Expression Profiling
Gene Expression Regulation, Plant
Host–pathogen interactions
Plant Diseases - genetics
Plant Diseases - immunology
Plant Diseases - virology
Plant Leaves - genetics
Plant Leaves - virology
Plant pathogens
Plant Viruses - pathogenicity
RNA silencing
RNA, Plant - analysis
Signal Transduction - genetics
Stress response
Viroids - pathogenicity
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Summary:Citrus are natural hosts of several viroids, which are plant pathogens composed exclusively of a non-protein-coding, small single-stranded circular RNA that is able to replicate autonomously in susceptible hosts. They are responsible for symptoms such as stunting, leaf epinasty, and chlorosis. Citrus viroid III (CVd-III) has been long regarded as a possible dwarfing agent of citrus grafted on trifoliate orange and its hybrids. To investigate molecular mechanisms involved in pathogenesis, the messenger RNA (mRNA) differential display technique was here applied to identify genes whose transcription was significantly altered in leaves of Etrog citron ( Citrus medica) infected by CVd-III (variant b). Of eighteen genes identified, thirteen were up-regulated by viroid infection, while five were down-regulated. Except for two genes that encode proteins of unknown function, the remaining genes are mainly involved in plant defence/stress responses, signal transduction, amino acid transport, and cell wall structure. Among the up-regulated genes, it is noteworthy a suppressor of RNA silencing that might be involved in viroid and virus pathogenicity. The functions of these genes are discussed.
ISSN:0167-4781
0006-3002
1879-2634
DOI:10.1016/j.bbaexp.2007.03.004