Cell-IQ visualization of motility, cell mass, and osteogenic differentiation of multipotent mesenchymal stromal cells cultured with relief calcium phosphate coating

The Cell-IQ continuous surveillance system allowed us to establish the following changes in a 14- day culture in vitro: a twofold suppression of the directional migration of multipotent mesenchymal stromal cells of human adipose tissue (MMSC-AT) towards the samples with a microarc calcium phosphate...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2017-09, Vol.476 (1), p.310-315
Main Authors: Litvinova, L. S., Shupletsova, V. V., Yurova, K. A., Khaziakhmatova, O. G., Todosenko, N. M., Khlusova, M. Yu, Slepchenko, G. B., Cherempey, E. G., Sharkeev, Yu. P., Komarova, E. G., Sedelnikova, M. B., Malashchenko, V. V., Melashchenko, E. S., Khlusov, I. A.
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose Tissue - cytology
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Calcium
Calcium phosphates
Calcium Phosphates - pharmacology
Cell culture
Cell Differentiation - drug effects
Cell Movement - drug effects
Cells
Cells, Cultured
Genetic transformation
Humans
Hydroxyapatite
Intelligence
Life Sciences
Mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchyme
Molecular Biology
Motility
Osteocalcin
Osteocalcin - metabolism
Osteogenesis - drug effects
Phosphate coatings
Phosphates
Phosphorus
Secretion
Stem cells
Stromal cells
Visualization
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Description
Summary:The Cell-IQ continuous surveillance system allowed us to establish the following changes in a 14- day culture in vitro: a twofold suppression of the directional migration of multipotent mesenchymal stromal cells of human adipose tissue (MMSC-AT) towards the samples with a microarc calcium phosphate (CP) coating from synthetic hydroxyapatite; a tenfold decrease in the cell mass on the interphase with the samples, which was accompanied by a slight reduction in the expression of membrane determinants of stromal stem cells; and an enhancement of their osteogenic differentiation (osteocalcin secretion and mineralized matrix formation) on the 21st day of the study. Calcium phosphate particles, but not the calcium and phosphorus ions, may trigger the phenotypic transformation of the MMSC-AT behavior in vitro.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672917050076