A color‐code for glycosaminoglycans identification by means of polyacrylamide gel electrophoresis stained with the cationic carbocyanine dye Stains‐all

Cationic dyes such as toluidin blue are commonly employed to visualize glycosaminoglycans (GAGs) on electrophoresis gels; however, the carbocyanine‐based dye Stains‐all have been increasingly used to stain the non‐sulfated hyaluronic acid and other GAGs in submicrogram quantities. In this short comm...

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Bibliographic Details
Published in:Electrophoresis 2018-02, Vol.39 (4), p.666-669
Main Authors: Andrade, João Pedro Souza, Oliveira, Caroline Pacheco, Tovar, Ana Maria Freire, Mourão, Paulo Antonio de Souza, Vilanova, Eduardo
Format: Article
Language:English
Subjects:
Carbocyanines - chemistry
Cationic dyes
Cations
Dyes
Electrophoresis
Electrophoresis, Polyacrylamide Gel - methods
Fluorescent Dyes - chemistry
Gels
Glycosaminoglycans
Glycosaminoglycans - analysis
Hyaluronic acid
Stains & staining
Stains‐all
Sulfated polysaccharides
Tolonium Chloride
Toluidine blue
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Summary:Cationic dyes such as toluidin blue are commonly employed to visualize glycosaminoglycans (GAGs) on electrophoresis gels; however, the carbocyanine‐based dye Stains‐all have been increasingly used to stain the non‐sulfated hyaluronic acid and other GAGs in submicrogram quantities. In this short communication, we demonstrate that Stains‐all is able to stain the most common GAGs on polyacrylamide gels with distinct and contrasting colors in a reproducible manner. We also show that this staining method is useful to identify GAGs present both in mixtures and in submicrogram quantities. Therefore, Stains‐all has shown to be useful in identifying GAGs on polyacrylamide gels with basis on their specific colors, at least on screening level.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201700391