Structural Characterization of a Tetrapeptide from Sesame Flavor-Type Baijiu and Its Preventive Effects against AAPH-Induced Oxidative Stress in HepG2 Cells

Peptides are rarely reported from Chinese Baijiu (Chinese liquor) because they are often present in very low concentrations in the complex matrix. A tetrapeptide, Ala-Lys-Arg-Ala (AKRA), was recently identified by high-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry...

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Published in:Journal of agricultural and food chemistry 2017-12, Vol.65 (48), p.10495-10504
Main Authors: Wu, Jihong, Huo, Jiaying, Huang, Mingquan, Zhao, Mouming, Luo, Xuelian, Sun, Baoguo
Format: Article
Language:English
Subjects:
Amidines - toxicity
Antioxidants - metabolism
Catalase - metabolism
Flavoring Agents - chemistry
Flavoring Agents - pharmacology
Glutathione - metabolism
Glutathione Peroxidase - metabolism
Hep G2 Cells
Humans
Mass Spectrometry
Oxidative Stress - drug effects
Peptides - chemistry
Peptides - pharmacology
Plant Extracts - chemistry
Plant Extracts - pharmacology
Reactive Oxygen Species - metabolism
Sesamum - chemistry
Superoxide Dismutase - metabolism
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Summary:Peptides are rarely reported from Chinese Baijiu (Chinese liquor) because they are often present in very low concentrations in the complex matrix. A tetrapeptide, Ala-Lys-Arg-Ala (AKRA), was recently identified by high-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry (HPLC-Q-TOF-MS) from sesame flavor-type Baijiu at a concentration of 8.497 ± 0.753 μg/L (P > 0.05), and this tetrapeptide showed preventive effects against 2,2′-azobis­(2-methylpropanimidamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells. The cellular antioxidant activity assay results showed that AKRA protected AAPH-induced oxidative stress in HepG2 cells in a concentration-dependent manner. Pretreatment of the cells for 2 h with AKRA (0.38–1.50 mg/mL) caused strong intracellular reactive oxygen species (ROS) scavenging activities and prevented a decrease in reduced glutathione (GSH) and increases in oxidized glutathione (GSSG) and malondialdehyde (MDA). In addition, AKRA treatment prevented significant decreases in glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) induced by AAPH. Thus, AKRA treatment ameliorated AAPH-induced oxidative stress in HepG2 cells. This study will be important for the design and regulation of functional Baijiu production.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.7b04815