No effect of topical application of tranexamic acid on articular cartilage

• Published in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA sports traumatology, arthroscopy : official journal of the ESSKA, 2019-03, Vol.27 (3), p.931-935
• Main Authors: Ambra, Luiz Felipe, de Girolamo, Laura, Niu, Wanting, Phan, Amy, Spector, Myron, Gomoll, Andreas H.
• Format: Article
• Language: English
• Subjects: Administration, Topical; Animals; Antifibrinolytic agents; Antifibrinolytic Agents - administration & dosage; Biocompatibility; Biomedical materials; Cartilage; Cartilage (articular); Cartilage, Articular - drug effects; Cell Survival - drug effects; Cell viability; Chondrocytes; Chondrocytes - drug effects; Cytotoxicity; Experimental Study; Explants; Exposure; Fluorescence; Knee; Medicine; Medicine & Public Health; Models, Animal; Orthopedics; Plugs; Saline solutions; Statistics; Surgery; Swine; Swine, Miniature; Topical application; Toxicity; Tranexamic Acid - administration & dosage
• ISSN: 0942-2056; 1433-7347
• DOI: 10.1007/s00167-017-4746-9

Purpose The objective of this study was to evaluate potential cytotoxicity of TXA on articular cartilage by assessing chondrocyte viability of osteochondral explants after exposure to different concentrations and durations of TXA. Methods Thirty-nine osteochondral plugs (OCPs) were harvested from three adult Yucatan minipigs immediately after their death. OCPs were divided into 13 groups exposed to different concentrations of TXA (1, 2 and 4 mg/ml in saline solution) for 1, 3 and 6 h. Negative controls were exposed to saline solution for 0, 1, 3 and 6 h. Chondrocyte viability was assessed by Live/Dead cell assay and calculated as the ratio of live cells (green fluorescence) to overall cells (green + red cells) for each concentration of TXA and time point in a 50-µm scanned image. Results No correlation was found between chondrocyte viability, and TXA concentration and time of exposure. Overall, chondrocyte viability ranged from 90 to 99%. There was no statistical difference among control group, 1, 2 and 4 mg/ml TXA solutions at each time point [1 h (n.s.), 3 h (n.s.), 6 h (n.s.)]. Similarly, no statistical difference among groups was observed when comparing cell viability at 1, 3 and 6 h of TXA exposure, (Fig. 2) [1 mg/ml (n.s.), 2 mg/ml (n.s.), and 4 mg/ml (n.s.)]. Conclusions In conclusion, doses of TXA approximating the current clinical protocols for topical use did not demonstrate any cytotoxic effects on cartilage explants in a Yucatan mini pig model. Thus, supporting the topical application for procedures with intact cartilage, such as partial knee replacement surgery and cartilage repair procedures.