Non-invasive detection of HPV DNA in exfoliative samples from ophthalmic pterygium: a feasibility study

Purpose The evaluation of a non-invasive detection method for human papilloma virus (HPV) in ophthalmic pterygia. Methods Cotton swab samples and corresponding tissue specimens were collected from 21 ophthalmic pterygia of 21 patients. HPV detection and typing were performed by real-time PCR. Discre...

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Bibliographic Details
Published in:Graefe's archive for clinical and experimental ophthalmology 2018, Vol.256 (1), p.193-198
Main Authors: Chalkia, Αikaterini K., Derdas, Stavros, Bontzos, Georgios, Sourvinos, George, Detorakis, Εfstathios T.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Conjunctiva - virology
Cotton
DNA, Viral - analysis
Eye Infections, Viral - complications
Eye Infections, Viral - diagnosis
Eye Infections, Viral - virology
Feasibility Studies
Female
Genotype
Genotypes
Human papillomavirus
Humans
Inflammatory Disorders
Male
Medicine
Medicine & Public Health
Middle Aged
Ophthalmology
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
Papillomavirus Infections - complications
Papillomavirus Infections - diagnosis
Papillomavirus Infections - virology
Polymerase chain reaction
Prospective Studies
Pterygium - complications
Pterygium - diagnosis
Real-Time Polymerase Chain Reaction
Statistical analysis
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Summary:Purpose The evaluation of a non-invasive detection method for human papilloma virus (HPV) in ophthalmic pterygia. Methods Cotton swab samples and corresponding tissue specimens were collected from 21 ophthalmic pterygia of 21 patients. HPV detection and typing were performed by real-time PCR. Discrepancies in HPV detection between swab and tissue samples as well as clinical correlations of findings were examined. Results HPV DNA was detected in 9 (42.86%) tissue specimens and 8 (38.09%) respective swab specimens. HPV genotypes 33, 39, 45, 56, 59 and 66 were identified in the examined specimens, while more than one strain's HPV type was detected in 2 specimens. HPV presence was significantly correlated with the female gender whereas other clinical associations were not statistically significant. Conclusions Findings imply that PCR-mediated HPV detection and typing in exfoliative swab specimens may be employed as a non-invasive diagnostic tool in the management of ophthalmic pterygia.
ISSN:0721-832X
1435-702X
DOI:10.1007/s00417-017-3840-5