Native hypersaline sulphate reducing bacteria contributes to iron nanoparticle formation in saltpan sediment: A concern for aquaculture

• Published in: Journal of environmental management 2018-01, Vol.206, p.556-564
• Main Authors: Das, Kirti Ranjan, Kowshik, Meenal, Praveen Kumar, M.K., Kerkar, Savita, Shyama, S.K., Mishra, Samir
• Format: Article
• Language: English
• Subjects: Biological nanoparticle; DNA damage; Hypersaline; Maghemite nanoparticle; Sulphate reducing bacteria; Zebra fish
• ISSN: 0301-4797; 1095-8630
• DOI: 10.1016/j.jenvman.2017.10.078

A hypersaline dissimilatory sulphate reducing bacterium, strain LS4, isolated from the sediments of Ribander saltpan, Goa, India was found to produce (Fe2O3) maghemite nanoparticles. The presence of maghemite nanoparticles was also detected in the same sediment. Strain LS4 was isolated anaerobically on modified Hatchikian's media at 300 psu, growing optimally at 30 °C, 150 psu salinity and pH 7.8. Based on biochemical characteristics and 16S rRNA sequence analysis, the strain LS4 belongs to genus Desulfovibrio. This isolate synthesized iron oxide nanoparticles in vitro when challenged with FeCl3 & FeSO4 in the growth medium. The biological nanoparticles were characterized to be Fe2O3 nanoparticle of 19 nm size by X-ray diffraction, transmission electron microscopy, fourier transform infrared spectroscopy, scanning electron microscopy and energy-dispersive x-ray spectroscopy. Maghemite nanoparticles (5.63 mg g−1) were isolated from the saltpan sediment by magnetic separation which showed similar characteristic features to the Fe2O3 nanoparticle produced by strain LS4 with an average size of 18 nm. Traditionally Goan saltpans were used for aquaculture during the non-salt making season, thus effects of these nanoparticles on Zebra fish embryo development were checked, which resulted in developmental abnormalities and DNA damage in a dose dependent manner. With the increasing nanoparticle concentration (0.1 mg.L−1 to100 mg.L−1), the mortality rate increased with a decrease in the hatching rate (93.05 ± 2.4 to 25 ± 4.16%) and heart rate (150–120 beats per minute). The nanoparticle exposed embryos developed malformed larvae with a characteristic of pericardial edema, curved body, curved notochord, curved tail and curved tail tip. These results suggest that strain LS4 might be playing a role as a contributor in the formation of iron oxide nanoparticle in the Ribander saltpan sediment, however; its high concentration will have a negative impact on aquaculture in these saltpans. [Display omitted] •Isolation of Fe2O3 nanoparticle producing hypersaline sulphate reducing bacteria•Bacteria contribute to nanoparticle formation in saltpan sediment.•Fe2O3 nanoparticle affects development of fish embryo.•High concentration of nanoparticle in saltpans not suitable for aquaculture use.