Trace determination of β-lactam antibiotics in environmental aqueous samples using off-line and on-line preconcentration in capillary electrophoresis

• Published in: Journal of Chromatography A 2008-03, Vol.1185 (2), p.273-280
• Main Authors: Bailón-Pérez, M.I., García-Campaña, A.M., Cruces-Blanco, C., del Olmo Iruela, M.
• Format: Article
• Language: English
• Subjects: Analysis methods; Analytical chemistry; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - chemistry; Applied sciences; beta-Lactams - analysis; beta-Lactams - chemistry; Capillary zone electrophoresis; Chemistry; Chromatographic methods and physical methods associated with chromatography; Electrophoresis, Capillary - methods; Environment; Environmental Monitoring - methods; Exact sciences and technology; Natural water pollution; Other chromatographic methods; Other wastewaters; Pollution; River water; Sample stacking; Sensitivity and Specificity; Solid Phase Extraction - methods; Wastewater; Wastewaters; Water - analysis; Water Pollutants, Chemical - analysis; Water treatment and pollution; Well water; β-Lactam antibiotics
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2007.12.088

A sensitive and reliable method using capillary zone electrophoresis with UV-diode array detection (CZE-DAD) has been developed and validated for trace determination of β-lactam antibiotics in waste, well and river water matrices. Due to the lack of sensitivity of the UV–vis detection, a solvent extraction/solid-phase extraction (SPE) method applied for off-line preconcentration and cleanup of water samples, in combination with an on-line preconcentration methodology named large volume sample stacking (LVSS) have been applied. The analytes included nafcillin, dicloxacillin, cloxacillin, oxacillin, ampicillin, penicillin G and amoxicillin. Average recoveries for water samples fortified with the studied β-lactams at different concentration levels (1.0, 2.0 and 4.0 μg/L) were ranging between 94 and 99%, with relative standard deviations (RSDs) lower than 10%. The precision, calculated as intra-day and inter-day standard deviations fell within acceptable ranges (3.3–7.2%). The limits of detection were estimated to range between 0.08 and 0.80 μg L −1 for the studied compounds. All the samples analyzed were negative for all the analytes at these levels of concentration and the method showed its usefulness for the detection of these widely applied β-lactam antibiotics in different kinds of waters.