Prospective role of indigenous Exiguobacterium profundum PT2 in arsenic biotransformation and biosorption by planktonic cultures and biofilms

• Published in: Journal of applied microbiology 2018-02, Vol.124 (2), p.431-443
• Main Authors: Saba, Andreasen, R., Li, Y., Rehman, Y., Ahmed, M., Meyer, R.L., Sabri, A.N.
• Format: Article
• Language: English
• Subjects: Adsorption; Arsenic; Arsenic - metabolism; arsenic biotransformation; Arsenic ions; arsenic resistance; Bacillaceae - chemistry; Bacillaceae - genetics; Bacillaceae - isolation & purification; Bacillaceae - metabolism; Bacteria; Biofilms; Biomass; bioremediation; Biosorption; Biotransformation; Culture; Gene sequencing; High-performance liquid chromatography; Industrial Waste - analysis; Industrial wastes; Industrial wastewater; Kinetics; Liquid chromatography; Nutrients; RNA, Ribosomal, 16S - metabolism; rRNA 16S; Waste Water - analysis; Wastewater; Wastewater treatment; Water Pollutants, Chemical - metabolism; Water treatment
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.13636

Aims The aim of this study was to analyse arsenic (As) transformation and biosorption by indigenous As‐resistant bacteria both in planktonic and biofilm modes of growth. Methods and Results As‐resistant bacteria were isolated from industrial waste water and strain PT2, and identified as Exiguobacterium profundum through 16S rRNA gene sequencing was selected for further study. As transformation and biosorption by E. profundumPT2 was determined by HPLC‐ICP‐MS analysis. Planktonic cultures reduced 3·73 mmol l−1 As5+ into As3+ from artificial waste water effluent after 48‐h incubation. In case of biosorption, planktonic cultures and biofilms exhibited 25·2 and 29·4 mg g−1 biomass biosorption, respectively. As biosorption kinetics followed Freundlich isotherm and pseudo second‐order model. Biofilm formation peaked after 3 days of incubation, and in the presence of As stress, biofilm formation was significantly affected in contrast to control (P