The making of a mushroom: Mitosis, nuclear migration and the actin network

[Display omitted] •Mitosis in haploid, monokaryotic cells revealed condensation of elongated nuclei.•Dikaryotic hyphae with synchronously dividing nuclei thereby keep nuclear identity.•Division of one nucleus is re-directed by actin networks towards the clamp cell.•Septation is preceded by actin pat...

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Bibliographic Details
Published in:Fungal genetics and biology 2018-02, Vol.111, p.85-91
Main Authors: Jung, Elke-Martina, Kothe, Erika, Raudaskoski, Marjatta
Format: Article
Language:English
Subjects:
Actin
Actins - physiology
Basidiomycetes
Cell Nucleus - physiology
Hyphae - cytology
Microscopy, Fluorescence
Microscopy, Video
Mitosis
Mitosis - physiology
Nuclear division
Schizophyllum - cytology
Schizophyllum commune
Septation
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Summary:[Display omitted] •Mitosis in haploid, monokaryotic cells revealed condensation of elongated nuclei.•Dikaryotic hyphae with synchronously dividing nuclei thereby keep nuclear identity.•Division of one nucleus is re-directed by actin networks towards the clamp cell.•Septation is preceded by actin patches and vesicles to form the actin ring. Basidiomycetes feature a prolonged dikaryotic life stage. A dispute over open versus closed mitosis could be solved using in vivo fluorescence videomicroscopy of histone 2B::EGFP and Lifeact labeled Schizophyllum commune. It revealed nuclei to condense to approximately one fifth in diameter during mitotic prophase. In addition, the specifics of clamp cell formation typical of many basidiomycetes included an actin network at the future site of nuclear division, which allowed for cessation of nuclear movement and re-localization of one nucleus towards the emerging clamp cell, while the other divided along the hyphal axis. Subsequent fusion of the clamp cell to form the clamp connection restored the close association of the two nuclei in a very fast process after clamp fusion. Septation was preceded by actin patches and vesicles involved in formation of the actin ring.
ISSN:1087-1845
1096-0937
DOI:10.1016/j.fgb.2017.11.001