Effects of the pH and Concentration on the Stability of Standard Solutions of Proteinogenic Amino Acid Mixtures

To prepare metrologically traceable amino acid mixed standard solutions, it is necessary to determine the stability of each amino acid present in the mixed solutions. In the present study, we prepared amino acid mixed solutions using certified reference standards of 17 proteinogenic amino acids, and...

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Bibliographic Details
Published in:Analytical Sciences 2017/11/10, Vol.33(11), pp.1241-1245
Main Authors: KATO, Megumi, YAMAZAKI, Taichi, KATO, Hisashi, YAMANAKA, Noriko, TAKATSU, Akiko, IHARA, Toshihide
Format: Article
Language:English
Subjects:
Accelerated tests
Amino acid mixed standard solution
Amino acids
Analytical Chemistry
certified reference material
Chemistry
Degradation
Glutamic acid
pH effects
preparation
Pyroglutamic acid
Stability
Stability analysis
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Summary:To prepare metrologically traceable amino acid mixed standard solutions, it is necessary to determine the stability of each amino acid present in the mixed solutions. In the present study, we prepared amino acid mixed solutions using certified reference standards of 17 proteinogenic amino acids, and examined the stability of each of these amino acids in 0.1 N HCl. We found that the concentration of glutamic acid decreased significantly during storage. LC/MS analysis indicated that the instability of glutamic acid was due to the partial degradation of glutamic acid to pyroglutamic acid in 0.1 N HCl. Using accelerated degradation tests, we investigated several solvent compositions to improve the stability of glutamic acid in amino acid mixed solution, and determined that the change of the pH by diluting the mixed solution improved the stability of glutamic acid.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.33.1241