PCR-based identification of clinically relevant Pseudallescheria/Scedosporium strains

Pseudallescheria boydii (anamorph: Scedosporium apiospermum) and S. prolificans can cause severe infections in both the immunocompromized host and accidentally injured people. A species-specific polymerase chain reaction (PCR) enabling detection and discrimination of these fungi has not been availab...

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Bibliographic Details
Published in:Medical mycology (Oxford) 1998-04, Vol.36 (2), p.61-67
Main Authors: WEDDE, M, MÜLLER, D, TINTELNOT, K, DE HOOG, G. S, STAHL, U
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Blotting, Southern
DNA, Ribosomal - genetics
Fundamental and applied biological sciences. Psychology
Humans
Immunocompromised Host
Microbiology
Molecular Sequence Data
Mycetoma - microbiology
Mycological methods and techniques used in mycology
Mycology
Phylogeny
Polymerase Chain Reaction - methods
Pseudallescheria - classification
Pseudallescheria - genetics
Pseudallescheria - isolation & purification
Pseudallescheria boydii
RNA, Ribosomal, 5.8S - genetics
Scedosporium apiospermum
Sequence Alignment
Sequence Homology, Nucleic Acid
Sewage
Soil Microbiology
Wounds and Injuries - microbiology
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Summary:Pseudallescheria boydii (anamorph: Scedosporium apiospermum) and S. prolificans can cause severe infections in both the immunocompromized host and accidentally injured people. A species-specific polymerase chain reaction (PCR) enabling detection and discrimination of these fungi has not been available to date. In view of the difficult treatment especially of S. prolificans infections, a PCR-based detection system has been developed. Based on results of partial sequencing of ribosomal DNA, Scedosporium DNA could be amplified, either at the genus or at the species level. Hybridization probes for the identification of the PCR-products were established.
ISSN:1369-3786
1460-2709
DOI:10.1046/j.1365-280x.1998.00130.x