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PCR-based identification of clinically relevant Pseudallescheria/Scedosporium strains
Pseudallescheria boydii (anamorph: Scedosporium apiospermum) and S. prolificans can cause severe infections in both the immunocompromized host and accidentally injured people. A species-specific polymerase chain reaction (PCR) enabling detection and discrimination of these fungi has not been availab...
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| Published in: | Medical mycology (Oxford) 1998-04, Vol.36 (2), p.61-67 |
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| Main Authors: | , , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c351t-90caa6b16c06d9e144c6694e11ff258ac82c6581f762713e7b8788a6e436d8273 |
| container_end_page | 67 |
| container_issue | 2 |
| container_start_page | 61 |
| container_title | Medical mycology (Oxford) |
| container_volume | 36 |
| creator | WEDDE, M MÜLLER, D TINTELNOT, K DE HOOG, G. S STAHL, U |
| description | Pseudallescheria boydii (anamorph: Scedosporium apiospermum) and S. prolificans can cause severe infections in both the immunocompromized host and accidentally injured people. A species-specific polymerase chain reaction (PCR) enabling detection and discrimination of these fungi has not been available to date. In view of the difficult treatment especially of S. prolificans infections, a PCR-based detection system has been developed. Based on results of partial sequencing of ribosomal DNA, Scedosporium DNA could be amplified, either at the genus or at the species level. Hybridization probes for the identification of the PCR-products were established. |
| doi_str_mv | 10.1046/j.1365-280x.1998.00130.x |
| format | article |
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| fulltext | fulltext |
| identifier | ISSN: 1369-3786 |
| ispartof | Medical mycology (Oxford), 1998-04, Vol.36 (2), p.61-67 |
| issn | 1369-3786 1460-2709 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19637881 |
| source | Oxford Journals Online |
| subjects | Base Sequence Biological and medical sciences Blotting, Southern DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Humans Immunocompromised Host Microbiology Molecular Sequence Data Mycetoma - microbiology Mycological methods and techniques used in mycology Mycology Phylogeny Polymerase Chain Reaction - methods Pseudallescheria - classification Pseudallescheria - genetics Pseudallescheria - isolation & purification Pseudallescheria boydii RNA, Ribosomal, 5.8S - genetics Scedosporium apiospermum Sequence Alignment Sequence Homology, Nucleic Acid Sewage Soil Microbiology Wounds and Injuries - microbiology |
| title | PCR-based identification of clinically relevant Pseudallescheria/Scedosporium strains |
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