Investigation into the effect of detergents on disinfectant susceptibility of attached Escherichia coli and Listeria monocytogenes

Investigate the effect of detergent treatment on susceptibility of attached Escherichia coli and Listeria monocytogenes to subsequent disinfectant treatment. Plate counts show that E. coli attached to stainless steel surfaces became significantly more susceptible to benzalkonium chloride (BAC) after...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied microbiology 2008-07, Vol.105 (1), p.309-315
Main Authors: Walton, J.T, Hill, D.J, Protheroe, R.G, Nevill, A, Gibson, H
Format: Article
Language:English
Subjects:
Bacterial Adhesion - drug effects
benzalkonium chloride
Benzalkonium Compounds - pharmacology
Biological and medical sciences
biological resistance
cell surface hydrophobicity
Colony Count, Microbial
detergent
Detergents - pharmacology
Disinfectants - pharmacology
Equipment Contamination - prevention & control
Escherichia coli
Escherichia coli - drug effects
Flow Cytometry
Food Microbiology
Fundamental and applied biological sciences. Psychology
Hydrophobic and Hydrophilic Interactions
Listeria monocytogenes
Listeria monocytogenes - drug effects
Microbial Sensitivity Tests
Microbiology
Stainless Steel
susceptibility
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Investigate the effect of detergent treatment on susceptibility of attached Escherichia coli and Listeria monocytogenes to subsequent disinfectant treatment. Plate counts show that E. coli attached to stainless steel surfaces became significantly more susceptible to benzalkonium chloride (BAC) after treatment with sodium alkyl sulfate (SAS) and fatty alcohol ethoxylate (FAE). No change in susceptibility was observed with Sodium dodecyl sulfate (SDS). L. monocytogenes became significantly less susceptible to BAC after treatment with SAS and SDS yet no change in susceptibility was observed with FAE. Flow cytometry using the fluoresceine propidium iodide revealed significant increases in cell membrane permeability of both organisms by SAS and FAE, although the effect was much greater in E. coli. No change was observed with SDS. Hydrophobic interaction chromatography showed that both organisms became less hydrophobic following treatment with SAS and SDS but FAE had no effect. In E. coli, detergents that increase susceptibility to BAC increase membrane permeability. In L. monocytogenes, detergents that reduce susceptibility to BAC lower cell surface hydrophobicity. Detergents can influence the sensitivity of pathogenic food borne micro-organisms to BAC.
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2008.03805.x