Sandwiched White Adipose Tissue: A Microphysiological System of Primary Human Adipose Tissue

White adipose tissue (WAT) is a critical organ in both health and disease. However, physiologically faithful tissue culture models of primary human WAT remain limited, at best. In this study we describe a novel WAT culture system in which primary human WAT is sandwiched between tissue-engineered she...

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Bibliographic Details
Published in:Tissue engineering. Part C, Methods Methods, 2018-03, Vol.24 (3), p.135-145
Main Authors: Lau, Frank H., Vogel, Kelly, Luckett, John P., Hunt, Maxwell, Meyer, Alicia, Rogers, Camille L., Tessler, Oren, Dupin, Charles L., St. Hilaire, Hugo, Islam, Kazi N., Frazier, Trivia, Gimble, Jeffrey M., Scahill, Steven
Format: Article
Language:English
Subjects:
Adipocytes
Adipocytes - cytology
Adipocytes - metabolism
Adipokines - metabolism
Adipose tissue
Adipose Tissue, White - cytology
Adipose Tissue, White - metabolism
Adipose Tissue, White - transplantation
Adult
Animal models
Animals
Body fat
Cell culture
Cell Differentiation
Female
Gene expression
Gene Expression Profiling
Health sciences
Humans
Lipolysis
Male
Medicine
Metabolism
Methods Articles
Mice
Middle Aged
Physiology
Precision medicine
Stem cells
Stromal cells
Stromal Cells - cytology
Stromal Cells - metabolism
Surgery
Tissue culture
Tissue Culture Techniques - methods
Tissue engineering
Tissue Engineering - methods
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Summary:White adipose tissue (WAT) is a critical organ in both health and disease. However, physiologically faithful tissue culture models of primary human WAT remain limited, at best. In this study we describe a novel WAT culture system in which primary human WAT is sandwiched between tissue-engineered sheets of adipose-derived stromal cells. This construct, called “sandwiched white adipose tissue” (SWAT), can be defined as a microphysiological system (MPS) since it is a tissue-engineered, multicellular, three-dimensional organ construct produced using human cells. We validated SWAT against the National Institutes of Health MPS standards and found that SWAT is viable in culture for 8 weeks, retains physiologic responses to exogenous signaling, secretes adipokines, and engrafts into animal models. These attributes position SWAT as a powerful tool for the study of WAT physiology, pathophysiology, personalized medicine, and pharmaceutical development.
ISSN:1937-3384
1937-3392
DOI:10.1089/ten.tec.2017.0339