Comparative cytotoxic and anti-tuberculosis activity of Aplysina caissara marine sponge crude extracts

Three crude extracts of Aplysina caissara, a marine sponge endemic to Brazil, were tested against a hepatoma cell line and Mycobacterium tuberculosis. The results demonstrate that all extracts are toxic and capable of inhibiting cellular growth. Additionally, the extracts produced morphological aber...

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Bibliographic Details
Published in:Comparative biochemistry and physiology. Toxicology & pharmacology 2008, Vol.147 (1), p.36-42
Main Authors: Azevedo, Luciana G., Muccillo-Baisch, Ana L., Filgueira, Daza de M.V.B., Boyle, Robert T., Ramos, Daniela F., Soares, Andrea D., Lerner, Clea, Silva, Pedro A., Trindade, Gilma S.
Format: Article
Language:English
Subjects:
Animals
Anti-tuberculosis assay
Antineoplastic Agents - isolation & purification
Antineoplastic Agents - pharmacology
Antitubercular Agents - isolation & purification
Antitubercular Agents - pharmacology
Aplysina caissara
Aplysina caissara marine sponge
Carcinoma, Hepatocellular - drug therapy
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - pathology
Cell Adhesion - drug effects
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
Cytotoxic assay
Dose-Response Relationship, Drug
Inhibitory Concentration 50
Liver Neoplasms - drug therapy
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
Marine
Mycobacterium tuberculosis
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - growth & development
Porifera - chemistry
Rats
Reactive Oxygen Species - metabolism
Time Factors
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Summary:Three crude extracts of Aplysina caissara, a marine sponge endemic to Brazil, were tested against a hepatoma cell line and Mycobacterium tuberculosis. The results demonstrate that all extracts are toxic and capable of inhibiting cellular growth. Additionally, the extracts produced morphological aberrations and inhibited cell attachment to culture substrates. These effects were dose/time dependent. Our results also suggest that reactive oxygen species (ROS) production is not involved in the cytotoxic processes levied by the extracts employed in this study and that active metabolites are likely to be present in the polar fractions of the crude extracts. Finally, our results indicate that all three extracts exhibit a moderate anti-tuberculosis capacity, and that the removal of an extract's lipid fraction appears to diminish this activity.
ISSN:1532-0456
1878-1659
DOI:10.1016/j.cbpc.2007.07.007