Micropropagation And Genetic Stability Of A Dendrobium Hybrid (Orchidaceae)

Dendrobium hybrids have great economic importance in a number of countries. Asymbiotic seed germination and the conventional vegetative method have been commonly used by growers to propagate these plants. To overcome somaclonal variation, which is commonly exhibited by Dendrobium (Nobile group) when...

Full description

Saved in:
Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2006-11, Vol.42 (6), p.568-571
Main Authors: Ferreira, WDM, Kerbauy, G B, Pimentel costa, AP
Format: Article
Language:English
Subjects:
Orchidaceae
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dendrobium hybrids have great economic importance in a number of countries. Asymbiotic seed germination and the conventional vegetative method have been commonly used by growers to propagate these plants. To overcome somaclonal variation, which is commonly exhibited by Dendrobium (Nobile group) when micropropagated from protocorm-like bodies, a protocol for propagating Dendrobium Second Love in vitro using axillary buds in the presence of thidiazuron was developed. Random amplified polymorphic DNA analysis was also carried out to check for possible genetic alterations in plants originating from six consecutive subcultures. The results revealed that the established protocol was efficient for the in vitro cloning of this orchid hybrid and the plants obtained from the six subcultures did not exhibit any type of polymorphism.
ISSN:1054-5476
DOI:10.1290/2006820.1