Heptad Repeats Regulate Protein Phosphatase 2A Recruitment to I-κB Kinase γ/NF-κB Essential Modulator and Are Targeted by Human T-lymphotropic Virus Type 1 Tax

• Published in: The Journal of biological chemistry 2007-04, Vol.282 (16), p.12119-12126
• Main Authors: Hong, Sohee, Wang, Ling-Chi, Gao, Xiang, Kuo, Yu-Liang, Liu, Baoying, Merling, Randall, Kung, Hsing-Jien, Shih, Hsiu-Ming, Giam, Chou-Zen
• Format: Article
• Language: English
• Subjects: Human T-lymphotropic virus; Human T-lymphotropic virus 1
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M610392200

The switching on-and-off of I-κB kinase (IKK) and NF-κB occurs rapidly after signaling. How activated IKK becomes down-regulated is not well understood. Here we show that following tumor necrosis factor-α stimulation, protein phosphatase 2A (PP2A) association with IKK is increased. A heptad repeat in IKKγ, helix 2 (HLX2), mediates PP2A recruitment. Two other heptad repeats downstream of HLX2, termed coiled-coil region 2 (CCR2) and leucine zipper (LZ), bind HLX2 and negatively regulate HLX2 interaction with PP2A. HTLV-1 transactivator Tax also binds HLX2, and this interaction is enhanced by CCR2 but reduced by LZ. In the presence of Tax, PP2A-IKKγ binding is greatly strengthened. Interestingly, peptides spanning CCR2 and/or LZ disrupt IKKγ-Tax and IKKγ-PP2A interactions and potently inhibit NF-κB activation by Tax and tumor necrosis factor-α. We propose that when IKK is resting, HLX2, CCR2, and LZ form a helical bundle in which HLX2 is sequestered. The HLX2-CCR2-LZ bundle becomes unfolded by signal-induced modifications of IKKγ or after Tax binding. In this conformation, IKK becomes activated. IKKγ then recruits PP2A via the exposed HLX2 domain for rapid down-regulation of IKK. Tax-PP2A interaction, however, renders PP2A inactive, thus maintaining Tax-PP2A-IKK in an active state. Finally, CCR2 and LZ possibly inhibit IKK activation by stabilizing the HLX2-CCR2-LZ bundle.