Relationships between soil pH and microbial properties in a UK arable soil

• Published in: Soil biology & biochemistry 2008-07, Vol.40 (7), p.1856-1861
• Main Authors: Aciego Pietri, J.C., Brookes, P.C.
• Format: Article
• Language: English
• Subjects: agricultural soils; Agronomy. Soil science and plant productions; aluminum; barley; Biochemistry and biology; Biological and medical sciences; biomass; calcium carbonate; carbon dioxide; carbon nitrogen ratio; chalk; Chemical, physicochemical, biochemical and biological properties; Extractable Al, Mn; Fundamental and applied biological sciences. Psychology; Hordeum vulgare; irrigated farming; linear models; manganese; microbial activity; Microbiology; nitrogen; Organic matter; Physics, chemistry, biochemistry and biology of agricultural and forest soils; silt loam soils; Soil C/N ratio; Soil microbial biomass; soil microorganisms; soil nutrients; soil organic carbon; soil organic matter; Soil pH; Soil pH gradient; Soil science; statistical analysis
• ISSN: 0038-0717; 1879-3428
• DOI: 10.1016/j.soilbio.2008.03.020

Effects of changing pH along a natural continuous gradient of a UK silty-loam soil were investigated. The site was a 200 m soil transect of the Hoosfield acid strip (Rothamsted Research, UK) which has grown continuous barley for more than 100 years. This experiment provides a remarkably uniform soil pH gradient, ranging from about pH 8.3 to 3.7. Soil total and organic C and the ratio: (soil organic C)/(soil total N) decreased due to decreasing plant C inputs as the soil pH declined. As expected, the CaCO 3 concentration was greatest at very high pH values (pH > 7.5). In contrast, extractable Al concentrations increased linearly ( R 2 = 0.94, p < 0.001) from below about pH 5.4, while extractable Mn concentrations were largest at pH 4.4 and decreased at lower pHs. Biomass C and biomass ninhydrin-N were greatest above pH 7. There were statistically significant relationships between soil pH and biomass C ( R 2 = 0.80, p < 0.001), biomass ninhydrin-N ( R 2 = 0.90, p < 0.001), organic C ( R 2 = 0.83, p < 0.001) and total N ( R 2 = 0.83, p < 0.001), confirming the importance of soil organic matter and pH in stimulating microbial biomass growth. Soil CO 2 evolution increased as pH increased ( R 2 = 0.97, p < 0.001). In contrast, the respiratory quotient ( qCO 2) had the greatest values at either end of the pH range. This is almost certainly a response to stress caused by the low p. At the highest pH, both abiotic (from CaCO 3) and biotic Co 2 will be involved so the effects of high pH on biomass activity are confounded. Microbial biomass and microbial activity tended to stabilise at pH values between about 5 and 7 because the differences in organic C, total N and Al concentrations within this pH range were small. This work has established clear relationships between microbial biomass and microbial activity over an extremely wide soil pH range and within a single soil type. In contrast, most other studies have used soils of both different pH and soil type to make similar comparisons. In the latter case, the effects of soil pH on microbial properties are confounded with effects of different soil types, vegetation cover and local climatic conditions.