Characterization of a novel single-stranded RNA mycovirus related to invertebrate viruses from the plant pathogen Verticillium dahliae

Fungal viruses, also known as mycoviruses, are widespread in all major groups of fungi. Mycoviruses from plant pathogens can reduce the virulence of their host fungus and have therefore potential as biological control agents. This has spurred the identification of novel mycoviruses in plant pathogen...

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Bibliographic Details
Published in:Archives of virology 2018-03, Vol.163 (3), p.771-776
Main Authors: Cañizares, M. Carmen, López-Escudero, Francisco J., Pérez-Artés, Encarnación, García-Pedrajas, María D.
Format: Article
Language:English
Subjects:
Annotated Sequence Record
Biological control
Biomedical and Life Sciences
Biomedicine
Fungi
Genomes
Host plants
Infectious Diseases
Medical Microbiology
Nucleotides
Open reading frames
Pathogens
Phylogeny
Plant viruses
RNA viruses
Verticillium dahliae
Verticillium wilt
Virology
Virulence
Viruses
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Summary:Fungal viruses, also known as mycoviruses, are widespread in all major groups of fungi. Mycoviruses from plant pathogens can reduce the virulence of their host fungus and have therefore potential as biological control agents. This has spurred the identification of novel mycoviruses in plant pathogens, research which is greatly contributing to our understanding of these organisms. In this work, we report the characterization of a novel monopartite mycovirus from Verticillium dahliae , the main causal agent of Verticillium wilt. This novel mycovirus, which we termed Verticillium dahliae RNA virus 1 (VdRV1), was identified in three different isolates of V. dahliae collected in olive growing areas of the Guadalquivir valley, southern Spain. We determined that the VdRV1 genome is a positive (+) single-stranded (ss) RNA, 2631 nucleotides in length, containing two open reading frames. VdRV1 showed few similarities with known mycoviruses, only with a group of unassigned (+) ssRNA mycoviruses which are related to plant viruses classified within the family Tombusviridae . However, phylogenetic analysis revealed that VdRV1 and the unassigned (+) ssRNA mycoviruses have a closer relationship with recently reported invertebrate viruses. This result indicates that as more viral sequences become available, the relationships of mycoviruses with viruses from other hosts should be reexamined. Additionally, the work supports the hypothesis of a heterogeneous origin for mycoviruses.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-017-3644-2