Surface sieving coordinated IMAC material for purification of His-tagged proteins

Tailor-made materials for the purification of proteins with His-tag was designed through synergizing the selectivity of surface sieving and metal ion affinity. By excluding impurity proteins out of the surface polymer network, such materials could purify His-tagged proteins from the crude cell lysis...

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Bibliographic Details
Published in:Analytica chimica acta 2018-01, Vol.997, p.9-15
Main Authors: Li, Senwu, Yang, Kaiguang, Liu, Lukuan, Zhao, Baofeng, Chen, Yuanbo, Li, Xiao, Zhang, Lihua, Zhang, Yukui
Format: Article
Language:English
Subjects:
Affinity
Chelation
His-tag
Lysis
Metal ion affinity
Metal ions
Metals
Polymers
Protein purification
Proteins
Purification
Surface sieving
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Summary:Tailor-made materials for the purification of proteins with His-tag was designed through synergizing the selectivity of surface sieving and metal ion affinity. By excluding impurity proteins out of the surface polymer network, such materials could purify His-tagged proteins from the crude cell lysis with purity up to 90%, improved by 14% compared to that obtained by the commercial metal chelating affinity materials. This study might promote the His-tagged protein purification to a new level. [Display omitted] •Novel IMAC material was prepared for one-step purification of His-tagged proteins.•Size sieving effect synergized metal ion affinity in this material.•RAFT was applied to precisely control the polymer shell for surface sieving.•High selectivity of target proteins could be achieved by such material.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2017.10.021