DNA Microviscosity Characterization with Particle Diffusometry for Downstream DNA Detection Applications

Analytical characterization of DNA microviscosity provides critical biophysical insights into nuclear crowding, nucleic acid based pharmaceutical development, and nucleic acid based biosensor device design. However, most viscosity characterization methods require large sample volumes and destructive...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2017-12, Vol.89 (24), p.13334-13341
Main Authors: Clayton, Katherine N, Berglund, Gregory D, Linnes, Jacqueline C, Kinzer-Ursem, Tamara L, Wereley, Steven T
Format: Article
Language:English
Subjects:
Algorithms
Amplification
Biosensors
Charge coupled devices
Chemistry
Deoxyribonucleic acid
Destructive testing
DNA
Fluorescence
Image processing
Klebsiella
Measurement
Neonates
Nondestructive testing
Pathogens
Sepsis
Topology
Viscosity
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Summary:Analytical characterization of DNA microviscosity provides critical biophysical insights into nuclear crowding, nucleic acid based pharmaceutical development, and nucleic acid based biosensor device design. However, most viscosity characterization methods require large sample volumes and destructive testing. In contrast, particle diffusometry permits in situ analysis of DNA microviscosity with short measurement times (8 s) using small volumes (
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.7b03513