Comparison of Somatic Embryogenesis in @@iMedicago sativa@ and @@iMedicago truncatula@

In this study, the regeneration through embryogenesis of two species of @@iMedicago@ were studied. Seeds of @@iMedicago sativa@ cv. Rehnani and @@iM. truncatula@ line A17 were grown on MS medium. After 4-6 weeks, segments of leaf and stem from two species were transferred to MS medium containing 2 m...

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Bibliographic Details
Published in:Pakistan journal of biological sciences 2007-02, Vol.10 (3), p.481-485
Main Authors: Hoori, F, Ehsanpour, A A, Mostajeran, A
Format: Article
Language:English
Subjects:
Medicago
Medicago sativa
Medicago truncatula
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Summary:In this study, the regeneration through embryogenesis of two species of @@iMedicago@ were studied. Seeds of @@iMedicago sativa@ cv. Rehnani and @@iM. truncatula@ line A17 were grown on MS medium. After 4-6 weeks, segments of leaf and stem from two species were transferred to MS medium containing 2 mg L@@u-1@ NAA, 2,4-D and Kinetin. The results indicated that callus formation from leaf explants of @@iM. sativa@ was higher than @@iM. trancatula.@ In the next stage, media with different combinations of auxin, cytokinin or ethinyl estradiol were provided for regeneration. Then in two stages, explants of leaf and stem of two species were transferred on these media. Results after 3-6 weeks showed that in medium containing NAA and TDZ, stem pieces of @@iM. sativa@ produced shoots while leaf pieces on NAA and ethinyl estradiol formed roots. Leaf explants of @@iM. truncatula@ in the medium containing NAA and BAP, produced somatic embryos. Also in media with auxin and ethinyl estradiol, somatic embryos were formed on calli of two species. Ethinyl estradiol and auxin together can induce somatic embryogenesis and root production on calli and stem or leaf explants.
ISSN:1028-8880