Faecal microbiota in dogs with multicentric lymphoma

Malignant lymphoma B‐cell type is the most common canine haematopoietic malignancy. Changes in intestinal microbiota have been implicated in few types of cancer in humans. The aim of this prospective and case‐control study was to determine differences in faecal microbiota between healthy control dog...

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Bibliographic Details
Published in:Veterinary & comparative oncology 2018-03, Vol.16 (1), p.E169-E175
Main Authors: Gavazza, A., Rossi, G., Lubas, G., Cerquetella, M., Minamoto, Y., Suchodolski, J. S.
Format: Article
Language:English
Subjects:
Animals
Case-Control Studies
DNA, Bacterial - genetics
dog
Dog Diseases - microbiology
Dogs
dysbiosis
Feces - microbiology
Female
Gastrointestinal Microbiome - genetics
lymphoma
Lymphoma - microbiology
Lymphoma - veterinary
Male
microbiota
Prospective Studies
qPCR analyses
Real-Time Polymerase Chain Reaction - veterinary
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA - veterinary
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Summary:Malignant lymphoma B‐cell type is the most common canine haematopoietic malignancy. Changes in intestinal microbiota have been implicated in few types of cancer in humans. The aim of this prospective and case‐control study was to determine differences in faecal microbiota between healthy control dogs and dogs with multicentric lymphoma. Twelve dogs affected by multicentric, B‐cell, stage III‐IV lymphoma, and 21 healthy dogs were enrolled in the study. For each dog, faecal samples were analysed by Illumina sequencing of 16S rRNA genes and quantitative PCR (qPCR) for selected bacterial groups. Alpha diversity was significant lower in lymphoma dogs. Principal coordinate analysis plots showed different microbial clustering (P = .001) and linear discriminant analysis effect size revealed 28 differentially abundant bacterial groups in lymphoma and control dogs. The qPCR analysis showed significant lower abundance of Faecalibacterium spp. (q 
ISSN:1476-5810
1476-5829
DOI:10.1111/vco.12367