Poly(L-lactic acid) nanofibers containing Cissus quadrangularis induced osteogenic differentiation in vitro

Cissus quadrangularis (CQ) is known as “bone setter” in Ayurvedic Medicine because of its ability to promote fracture healing. Polymers incorporated with CQ at lower concentration have shown to enhance osteogenic differentiation of mesenchymal stem cells (MSCs) in vitro. However, for the healing of...

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Bibliographic Details
Published in:International journal of biological macromolecules 2018-04, Vol.110, p.514-521
Main Authors: Parvathi, K, Krishnan, Amit G., Anitha, A, Jayakumar, R., Nair, Manitha B.
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Cissus - chemistry
Cissus quadrangularis
Electrospinning
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Nanofibers - chemistry
Osteogenesis
Osteoinduction
Polyesters - chemistry
Rats
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Summary:Cissus quadrangularis (CQ) is known as “bone setter” in Ayurvedic Medicine because of its ability to promote fracture healing. Polymers incorporated with CQ at lower concentration have shown to enhance osteogenic differentiation of mesenchymal stem cells (MSCs) in vitro. However, for the healing of clinically relevant critical sized bone defects, large amount of CQ would be required. Based on this perception, a herbal fibrous sheet containing high weight percentage of CQ [20,40 and 60wt/wt% in poly (L-lactic acid) (PLLA)] was fabricated through electrospinning. The solution concentration, flow rate, voltage and tip-target distance was optimized to obtain nanofibers. The hydrophobicity of PLLA fibers was reduced through CQ incorporation. There was considerable increase in the adhesion, proliferation and osteogenic differentiation of MSCs on herbal fibers than normal fibers, mainly on P-Q20 and P-CQ40. MSCs were differentiated into osteoblasts without providing any osteogenic supplements in the medium, indicating its osteoinductive capability. The herbal sheet also could promote mineralization when immersed in simulated body fluid for 14days. These studies specify that PLLA nanofibers loaded with 20 and 40wt% of CQ could serve as a potential candidate for bone tissue engineering applications.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2017.11.094