GV1001 Induces Apoptosis by Reducing Angiogenesis in Renal Cell Carcinoma Cells Both In Vitro and In Vivo

• Published in: Urology (Ridgewood, N.J.) N.J.), 2018-03, Vol.113, p.129-137
• Main Authors: Kim, Ga Eun, Jung, Ae Ryang, Kim, Mee Young, Lee, Joseph Bada, Im, Ji Houn, Lee, Kyu Won, Park, Yong Hyun, Lee, Ji Youl
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; Carcinoma, Renal Cell - drug therapy; Carcinoma, Renal Cell - pathology; Cell Line, Tumor - drug effects; Cell Proliferation - drug effects; Cell Survival - drug effects; Disease Models, Animal; Heterografts; Humans; In Situ Nick-End Labeling; In Vitro Techniques; Kidney Neoplasms - drug therapy; Kidney Neoplasms - pathology; Mice; Neoplasms, Experimental - drug therapy; Neovascularization, Pathologic - prevention & control; Peptide Fragments - pharmacology; Sensitivity and Specificity; Telomerase - pharmacology; Treatment Outcome
• ISSN: 0090-4295; 1527-9995
• DOI: 10.1016/j.urology.2017.10.038

To investigate the anticancer effects of GV1001 and its biological mechanism of action in renal cell carcinoma (RCC). The effects of GV1001 on cell survival and apoptosis in RCC cells were examined in vitro using cell viability assay, fluorescence-activated cell sorting, and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. To evaluate the effect of GV1001 on migration, invasion, and angiogenesis, we used wound healing, invasion, endothelial cell tube formation assay, and western blot analysis. Furthermore, we used an RCC xenograft model with either phosphate buffered saline or GV1001 to confirm the anticancer effect of GV1001 in vivo. Tumor volume was monitored during treatment, and tumor weight was measured after animals were killed. Apoptosis and angiogenesis of the tumor tissue were assessed using hematoxylin and eosin staining, immunohistochemistry, and western blot analysis. GV1001 reduced cell viability and induced apoptosis in RCC cells in vitro. Furthermore, GV1001 suppressed the migration and invasion of RCC cells through regulation of matrix metalloproteinases and tissue inhibitors of metalloproteinases. In addition, GV1001 reduced angiogenesis via regulation of hypoxia-inducible factor 1α. In xenograft mouse model experiment, GV1001 reduced tumor growth and induced apoptosis. As in the in vitro results, GV1001 significantly reduced angiogenesis through regulation of hypoxia-inducible factor 1α in vivo. Our data demonstrated that GV1001 induced apoptosis through suppression of angiogenesis in RCCs both in vitro and in vivo, which suggests that GV1001 may be a potential therapeutic target for RCC.