Noninvasive, high‐speed, near‐infrared imaging of the biomolecular distribution and molecular mechanism of embryonic development in fertilized fish eggs

• Published in: Journal of biophotonics 2018-04, Vol.11 (4), p.e201700115-n/a
• Main Authors: Ishigaki, Mika, Nishii, Takashi, Puangchit, Paralee, Yasui, Yui, Huck, Christian W., Ozaki, Yukihiro
• Format: Article
• Language: English
• Subjects: Biological effects; Biomaterials; Biomedical materials; Biomolecules; Eggs; Embryogenesis; Embryonic growth stage; Embryos; Fatty acids; Fish; fish egg; Fish eggs; Infrared analysis; Infrared imaging; interface; Lipid metabolism; Lipids; Lipoproteins; Membranes; Metabolism; near‐infrared (NIR) imaging; noninvasive analysis; Peptide mapping; Proteins; water
• ISSN: 1864-063X; 1864-0648
• DOI: 10.1002/jbio.201700115

In this study, the distribution of biomaterials and its molecular mechanism of embryonic development in Japanese medaka fish were analyzed nondestructively and noninvasively without staining using near‐infrared (NIR) imaging. The microscopic NIR imaging system used in this research was a device capable of ultra‐high‐speed imaging; using this system, one can acquire microscopic imaging data in a few seconds. Therefore, the medaka eggs remained alive throughout measurements and were successfully monitored in vivo. The distributions of biomolecules were examined by mapping the intensities of NIR bands resulting from lipids, proteins and water in 2 dimensions (2D). The structures of eyes, lipid bilayer membranes, micelles and water‐structure differences at the interface of different substances constituting different structures on the egg were visualized. Furthermore, insights on the metabolic mechanisms of lipids and membrane functions were drawn from the biased distribution of lipoproteins and the presence of unsaturated fatty acids in the egg membrane. These results indicated the potential for NIR imaging in evaluating the biological functions and metabolic systems of cells and embryos. The biomaterial distribution and its molecular mechanism of embryonic development in Japanese medaka fish were visualized without staining using high‐speed near‐infrared (NIR) imaging. It was a remarkable achievement to visualize the structures of eyes, lipid bilayer membranes, micelles and water‐structure differences at the interface of different substances constituting different structures on the egg. Furthermore, insights on the metabolic mechanisms of lipids and membrane functions were drawn from the biased distribution of lipoproteins and the presence of unsaturated fatty acids in the egg membrane.