Native promoters of Corynebacterium glutamicum and its application in l-lysine production

• Published in: Biotechnology letters 2018-02, Vol.40 (2), p.383-391
• Main Authors: Shang, Xiuling, Chai, Xin, Lu, Xuemei, Li, Yuan, Zhang, Yun, Wang, Guoqiang, Zhang, Chen, Liu, Shuwen, Zhang, Yu, Ma, Jiyin, Wen, Tingyi
• Format: Article
• Language: English
• Subjects: Applied Microbiology; beta-Galactosidase - genetics; beta-Galactosidase - metabolism; Biochemistry; Biomedical and Life Sciences; Biosynthesis; Biotechnology; Conserved sequence; Corynebacterium glutamicum; Corynebacterium glutamicum - genetics; Corynebacterium glutamicum - metabolism; DNA, Bacterial; Enzymatic activity; Galactosidase; Gene expression; Gene Expression Regulation, Bacterial - genetics; Genes, Reporter - genetics; Gram-positive bacteria; Life Sciences; Lysine; Lysine - metabolism; Metabolic engineering; Metabolic Engineering - methods; Metabolism; Microbiology; Original Research Paper; Promoter Regions, Genetic - genetics; Promoters; Reporter gene; Succinyl-CoA; β-Galactosidase
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-017-2479-y

Objective To identify useful native promoters of Corynebacterium glutamicum for fine-tuning of gene expression in metabolic engineering. Results Sixteen native promoters of C. glutamicum were characterized. These promoters covered a strength range of 31-fold with small increments and exhibited relatively stable activity during the whole growth phase using β-galactosidase as the reporter. The mRNA level and enzymatic activity of the lacZ reporter gene exhibited high correlation ( R 2  = 0.96) under the control of these promoters. Sequence analysis found that strong promoters had high similarity of the -10 hexamer to the consensus sequence and preference of the AT-rich UP element upstream the -35 region. To test the utility of the promoter library, the characterized native promoters were applied to modulate the sucCD -encoded succinyl-CoA synthetase expression for l -lysine overproduction. Conclusions The native promoters with various strengths realize the efficient and precise regulation of gene expression in metabolic engineering of C. glutamicum .