Genetic Transformation System for Members of the Genera, Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis

This paper describes a plasmid transformation system that permits genetic manipulation of the genus Sphingomonas. A cryptic indigenous plasmid, pAMI-1, of 10 kb from Sphingobium amiense was isolated and characterized. Nucleotide sequencing revealed that pAMI-1 contains five open reading frames, whic...

Full description

Saved in:
Bibliographic Details
Published in:Microbes and Environments 2006, Vol.21(4), pp.235-239
Main Authors: Saito, Miyuki, Ikunaga, Yoko, Ohta, Hiroyuki, Kurusu, Yasurou
Format: Article
Language:English
Subjects:
Escherichia
Novosphingobium
plasmid
Sphingobium
Sphingomonas
Sphingopyxis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This paper describes a plasmid transformation system that permits genetic manipulation of the genus Sphingomonas. A cryptic indigenous plasmid, pAMI-1, of 10 kb from Sphingobium amiense was isolated and characterized. Nucleotide sequencing revealed that pAMI-1 contains five open reading frames, which are predicted to encode proteins associated with integration, recombination, conjugation and replication. Escherichia coli-S. amiense shuttle vectors were successfully introduced into Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis strains by electroporation. The copy number of the shuttle vector was estimated to be 1-2 per chromosome in the Sphingobium yanoikuyae cell.
ISSN:1342-6311
1347-4405
DOI:10.1264/jsme2.21.235