Effects of Bacillus subtilis on some physiological and biochemical parameters of Triticum aestivum L. (wheat) under salinity

• Published in: Plant physiology and biochemistry 2017-12, Vol.121, p.80-88
• Main Authors: Lastochkina, Oksana, Pusenkova, Ludmila, Yuldashev, Ruslan, Babaev, Marat, Garipova, Svetlana, Blagova, Dar'ya, Khairullin, Ramil, Aliniaeifard, Sasan
• Format: Article
• Language: English
• Subjects: Bacillus subtilis; Bacillus subtilis - physiology; Malondialdehyde; Osmotic Pressure; Plant Root Cap - metabolism; proline; Salicylic acid; Salinity; Seedlings - metabolism; Seedlings - microbiology; Triticum - metabolism; Triticum - microbiology; Triticum aestivum L; Water storage capacity
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2017.10.020

Endophytic strain Bacillus subtilis (B. subtilis) 10–4, producing indole-3-acetic acid (IAA) and siderofores but not active in phosphate solubilization, exerted a protective effect on Triticum aestivum L. (wheat) plant grown under salinity (2% NaCl) stress. Exposure to salt stress resulted in an essential increase of proline (Pro) and malondialdehyde (MDA) level in the seedlings. At the same time the seedlings inoculated with B. subtilis 10-4 were characterized by decreased level of stress-induced Pro and MDA accumulation. It was revealed that both B. subtilis 10-4 and salinity caused increase in the content of endogenous salicylic acid (SA) in wheat seedlings as compared to SA content in the control, while B. subtilis 10-4 suppressed stress-induced SA accumulation. Water storage capacity (WSC) in leaf tissues was increased and stress-induced hydrolysis of statolite starch in root cap cells of the germinal roots was reduced by B. subtilis 10-4. The obtained data indicated that the activation of the defense reactions induced by B. subtilis 10-4 induced defense reactions may be connected with their ability to decrease the level of stress-induced oxidative and osmotic stress in seedlings and with the increase of endogenous SA level that can make a significant contribution to the implementation of the protective effect of B. subtilis 10-4 and is manifested in the improvement of plant growth, WSC of leaves and slowing down of the process of statolite starch hydrolysis under salinity. •Endophytic B. subtilis 10-4 exerts a protective effect on Triticum aestivum L. (wheat) plant growth under salinity (NaCl).•B. subtilis 10-4 decreases the levels of stress-induced oxidative and osmotic stress in seedlings.•B. subtilis 10-4 increases WSC in wheat leaves and reduces NaCl-induced hydrolysis of statolite starch in germinal roots.•B. subtilis 10-4 increases the endogenous SA level in wheat seedlings.•Involvement of SA-signaling pathway in B. subtilis 10-4-induced protective reactions on wheat under salinity was suggested.