OECD validation of the rodent Hershberger assay using three reference chemicals; 17α-methyltestosterone, procymidone, and p, p'-DDE

The rodent Hershberger assay is being validated as an in vivo test method for detecting androgenic or antiandrogenic compounds by the Organization for Economic Cooperation and Development (OECD). As part of the international validation work, we studied 17α-methyltestosterone for evaluating androgeni...

Full description

Saved in:
Bibliographic Details
Published in:Archives of toxicology 2007-05, Vol.81 (5), p.309-318
Main Authors: SHIN, Jae-Ho, HYUN JU MOON, IL HYUN KANG, TAE SUNG KIM, SU JUNG LEE, JI YOUN AHN, BAE, Hoon, EUI BAE JEUNG, SOON YOUNG HAN
Format: Article
Language:English
Subjects:
Antiandrogenic activity
Assaying
Biocompatibility
Biological and medical sciences
Biomedical materials
Castration
Chemicals
DDE
In vivo methods and tests
Medical sciences
Methyltestosterone
Nitrous oxide
Pesticides, fertilizers and other agrochemicals toxicology
Procymidone
Propionic acid
Rodents
Sex
Testosterone
Testosterone propionate
Toxicity
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The rodent Hershberger assay is being validated as an in vivo test method for detecting androgenic or antiandrogenic compounds by the Organization for Economic Cooperation and Development (OECD). As part of the international validation work, we studied 17α-methyltestosterone for evaluating androgenic activity, and procymidone and p,p′-DDE for evaluating antiandrogenic activity. Male Sprague–Dawley rats were castrated at postnatal day 42, and only the rats that showed preputial separation were used in this study. Seven days after castration, chemicals were administered daily by gavages to groups of rats for 10 days, as recommended by OECD phase-2 protocol. Administration of 17α-methyltestosterone induced increases of weights of accessory sex tissues and glands in a dose-dependent manner. Administration of procymidone and p,p′-DDE produced a dose-dependent decrease of weights of accessory sex tissues and glands in the rats co-treated with testosterone propionate (0.4 mg/kg/day) subcutaneously. Our data strongly suggested that the current protocol of OECD Hershberger assay (phase-2) should be used as a reliable method for the detection of endocrine related toxicity of other chemicals.
ISSN:0340-5761
1432-0738
DOI:10.1007/s00204-006-0174-1