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Shedding of food-borne pathogens and microbiological carcass contamination in rabbits at slaughter
To obtain microbiological data from rabbits at slaughter, 500 fecal samples and 500 carcasses samples were examined. All samples tested negative for Listeria and Salmonella. Campylobacter were detected in two fecal samples. Of the 500 fecal samples, 45.8% tested positive for eae (intimin), 1.2% for...
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| Published in: | Veterinary microbiology 2008-11, Vol.132 (1), p.149-157 |
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| creator | Kohler, R. Krause, G. Beutin, L. Stephan, R. Zweifel, C. |
| description | To obtain microbiological data from rabbits at slaughter, 500 fecal samples and 500 carcasses samples were examined. All samples tested negative for
Listeria and
Salmonella.
Campylobacter were detected in two fecal samples. Of the 500 fecal samples, 45.8% tested positive for
eae (intimin), 1.2% for
stx (Shiga toxin), and 1.8% for both
eae and
stx. By colony hybridization, 56
eae positive
Escherichia coli strains were isolated. Among them, 27 strains (48.2%) were of the serotypes O178:H7 and O153:H7, whereas 15 strains (26.8%) belonged to a serogroup that has not yet been described (O(CB10681):H7). All strains possessed intimin β1 and the translocated intimin receptor (
tir) capable of being tyrosine phosphorylated. None of the strains harbored the genes for Shiga toxins, EAST1 (
astA), bundlin (
bfpA), or the EAF plasmid. Slaughter rabbits therefore constitute a reservoir for certain atypical enteropathogenic
Escherichia coli. On rabbit carcasses, average total bacterial counts accounted for 3.3
log
CFU
cm
−2.
Enterobacteriaceae and coagulase positive staphylococci (CPS) were detected on 118 (23.6%) and 153 (30.6%) carcasses, respectively.
Enterobacteriaceae and CPS counts of positive samples were mainly |
| doi_str_mv | 10.1016/j.vetmic.2008.04.020 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19686700</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378113508001533</els_id><sourcerecordid>19686700</sourcerecordid><originalsourceid>FETCH-LOGICAL-c491t-c7abd9ea0ce9d376b6b4b2f3b7f0639e2b9da58c76538a2d30315fff2648e12a3</originalsourceid><addsrcrecordid>eNp9kcuO1DAQRS0EYpqBP0DgDewSynbiJBskNOIljcRimLVVfqXdStuNnR6Jv8ejtGDHqjbn1uMUIa8ZtAyY_HBoH9x6DKblAGMLXQscnpAdGwfR8L7jT8kOxDA2jIn-irwo5QAA3SThObliY8-6Tow7ou_2ztoQZ5o89SnZRqccHT3huk-zi4VitLSOyUmHtKQ5GFyowWywFGpSXPEYIq4hRRoizah1WGtopWXB87xfXX5Jnnlcint1qdfk_svnnzffmtsfX7_ffLptTDextTEDajs5BOMmKwappe4090IPHqSYHNeTxX40g-zFiNwKEKz33nPZjY5xFNfk_db3lNOvsyurOoZi3LJgdOlcFJvkKAeACnYbWI8qJTuvTjkcMf9WDNSjW3VQm1v16FZBp6rbGntz6X_WR2f_hS4yK_DuAmCplnzGaEL5y3EY6gKSVe7txnlMCudcmfs7DkwAq39jklfi40a46ushuKyKCS4aZ0N2ZlU2hf_v-gfY86Rn</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19686700</pqid></control><display><type>article</type><title>Shedding of food-borne pathogens and microbiological carcass contamination in rabbits at slaughter</title><source>ScienceDirect Freedom Collection</source><creator>Kohler, R. ; Krause, G. ; Beutin, L. ; Stephan, R. ; Zweifel, C.</creator><creatorcontrib>Kohler, R. ; Krause, G. ; Beutin, L. ; Stephan, R. ; Zweifel, C.</creatorcontrib><description>To obtain microbiological data from rabbits at slaughter, 500 fecal samples and 500 carcasses samples were examined. All samples tested negative for
Listeria and
Salmonella.
Campylobacter were detected in two fecal samples. Of the 500 fecal samples, 45.8% tested positive for
eae (intimin), 1.2% for
stx (Shiga toxin), and 1.8% for both
eae and
stx. By colony hybridization, 56
eae positive
Escherichia coli strains were isolated. Among them, 27 strains (48.2%) were of the serotypes O178:H7 and O153:H7, whereas 15 strains (26.8%) belonged to a serogroup that has not yet been described (O(CB10681):H7). All strains possessed intimin β1 and the translocated intimin receptor (
tir) capable of being tyrosine phosphorylated. None of the strains harbored the genes for Shiga toxins, EAST1 (
astA), bundlin (
bfpA), or the EAF plasmid. Slaughter rabbits therefore constitute a reservoir for certain atypical enteropathogenic
Escherichia coli. On rabbit carcasses, average total bacterial counts accounted for 3.3
log
CFU
cm
−2.
Enterobacteriaceae and coagulase positive staphylococci (CPS) were detected on 118 (23.6%) and 153 (30.6%) carcasses, respectively.
Enterobacteriaceae and CPS counts of positive samples were mainly <1.5
log
CFU
cm
−2. Among 153 selected CPS isolates, 98.7% were identified as
Staphylococcus aureus. None of the 151 isolated strains harbored the gene for methicillin resistance (
mecA). Genes for staphylococcal enterotoxins (SE) were detected in 102 strains. The combinations of
seg and
sei (53 strains) and
sed,
seg,
sei, and
sej (27 strains) dominated.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2008.04.020</identifier><identifier>PMID: 18514438</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Abattoirs ; animal pathogenic bacteria ; animal production ; Animals ; Bacteria - classification ; Bacteria - isolation & purification ; bacterial contamination ; bacterial toxins ; Bacteriology ; Biological and medical sciences ; Campylobacter ; Carcass contamination ; disease reservoirs ; Enterobacteriaceae ; Enteropathogenic Escherichia coli ; epidemiological studies ; Escherichia coli ; Fecal shedding ; feces ; Feces - microbiology ; food animals ; Food Microbiology ; food pathogens ; foodborne illness ; Fundamental and applied biological sciences. Psychology ; human diseases ; human food chain ; Listeria ; meat carcasses ; meat inspection ; microbiological quality ; Microbiology ; Miscellaneous ; Rabbit ; rabbits ; Rabbits - microbiology ; risk assessment ; Salmonella ; Staphylococcus aureus</subject><ispartof>Veterinary microbiology, 2008-11, Vol.132 (1), p.149-157</ispartof><rights>2008 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-c7abd9ea0ce9d376b6b4b2f3b7f0639e2b9da58c76538a2d30315fff2648e12a3</citedby><cites>FETCH-LOGICAL-c491t-c7abd9ea0ce9d376b6b4b2f3b7f0639e2b9da58c76538a2d30315fff2648e12a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20768661$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18514438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kohler, R.</creatorcontrib><creatorcontrib>Krause, G.</creatorcontrib><creatorcontrib>Beutin, L.</creatorcontrib><creatorcontrib>Stephan, R.</creatorcontrib><creatorcontrib>Zweifel, C.</creatorcontrib><title>Shedding of food-borne pathogens and microbiological carcass contamination in rabbits at slaughter</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>To obtain microbiological data from rabbits at slaughter, 500 fecal samples and 500 carcasses samples were examined. All samples tested negative for
Listeria and
Salmonella.
Campylobacter were detected in two fecal samples. Of the 500 fecal samples, 45.8% tested positive for
eae (intimin), 1.2% for
stx (Shiga toxin), and 1.8% for both
eae and
stx. By colony hybridization, 56
eae positive
Escherichia coli strains were isolated. Among them, 27 strains (48.2%) were of the serotypes O178:H7 and O153:H7, whereas 15 strains (26.8%) belonged to a serogroup that has not yet been described (O(CB10681):H7). All strains possessed intimin β1 and the translocated intimin receptor (
tir) capable of being tyrosine phosphorylated. None of the strains harbored the genes for Shiga toxins, EAST1 (
astA), bundlin (
bfpA), or the EAF plasmid. Slaughter rabbits therefore constitute a reservoir for certain atypical enteropathogenic
Escherichia coli. On rabbit carcasses, average total bacterial counts accounted for 3.3
log
CFU
cm
−2.
Enterobacteriaceae and coagulase positive staphylococci (CPS) were detected on 118 (23.6%) and 153 (30.6%) carcasses, respectively.
Enterobacteriaceae and CPS counts of positive samples were mainly <1.5
log
CFU
cm
−2. Among 153 selected CPS isolates, 98.7% were identified as
Staphylococcus aureus. None of the 151 isolated strains harbored the gene for methicillin resistance (
mecA). Genes for staphylococcal enterotoxins (SE) were detected in 102 strains. The combinations of
seg and
sei (53 strains) and
sed,
seg,
sei, and
sej (27 strains) dominated.</description><subject>Abattoirs</subject><subject>animal pathogenic bacteria</subject><subject>animal production</subject><subject>Animals</subject><subject>Bacteria - classification</subject><subject>Bacteria - isolation & purification</subject><subject>bacterial contamination</subject><subject>bacterial toxins</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Campylobacter</subject><subject>Carcass contamination</subject><subject>disease reservoirs</subject><subject>Enterobacteriaceae</subject><subject>Enteropathogenic Escherichia coli</subject><subject>epidemiological studies</subject><subject>Escherichia coli</subject><subject>Fecal shedding</subject><subject>feces</subject><subject>Feces - microbiology</subject><subject>food animals</subject><subject>Food Microbiology</subject><subject>food pathogens</subject><subject>foodborne illness</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>human diseases</subject><subject>human food chain</subject><subject>Listeria</subject><subject>meat carcasses</subject><subject>meat inspection</subject><subject>microbiological quality</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Rabbit</subject><subject>rabbits</subject><subject>Rabbits - microbiology</subject><subject>risk assessment</subject><subject>Salmonella</subject><subject>Staphylococcus aureus</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9kcuO1DAQRS0EYpqBP0DgDewSynbiJBskNOIljcRimLVVfqXdStuNnR6Jv8ejtGDHqjbn1uMUIa8ZtAyY_HBoH9x6DKblAGMLXQscnpAdGwfR8L7jT8kOxDA2jIn-irwo5QAA3SThObliY8-6Tow7ou_2ztoQZ5o89SnZRqccHT3huk-zi4VitLSOyUmHtKQ5GFyowWywFGpSXPEYIq4hRRoizah1WGtopWXB87xfXX5Jnnlcint1qdfk_svnnzffmtsfX7_ffLptTDextTEDajs5BOMmKwappe4090IPHqSYHNeTxX40g-zFiNwKEKz33nPZjY5xFNfk_db3lNOvsyurOoZi3LJgdOlcFJvkKAeACnYbWI8qJTuvTjkcMf9WDNSjW3VQm1v16FZBp6rbGntz6X_WR2f_hS4yK_DuAmCplnzGaEL5y3EY6gKSVe7txnlMCudcmfs7DkwAq39jklfi40a46ushuKyKCS4aZ0N2ZlU2hf_v-gfY86Rn</recordid><startdate>20081125</startdate><enddate>20081125</enddate><creator>Kohler, R.</creator><creator>Krause, G.</creator><creator>Beutin, L.</creator><creator>Stephan, R.</creator><creator>Zweifel, C.</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20081125</creationdate><title>Shedding of food-borne pathogens and microbiological carcass contamination in rabbits at slaughter</title><author>Kohler, R. ; Krause, G. ; Beutin, L. ; Stephan, R. ; Zweifel, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-c7abd9ea0ce9d376b6b4b2f3b7f0639e2b9da58c76538a2d30315fff2648e12a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Abattoirs</topic><topic>animal pathogenic bacteria</topic><topic>animal production</topic><topic>Animals</topic><topic>Bacteria - classification</topic><topic>Bacteria - isolation & purification</topic><topic>bacterial contamination</topic><topic>bacterial toxins</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Campylobacter</topic><topic>Carcass contamination</topic><topic>disease reservoirs</topic><topic>Enterobacteriaceae</topic><topic>Enteropathogenic Escherichia coli</topic><topic>epidemiological studies</topic><topic>Escherichia coli</topic><topic>Fecal shedding</topic><topic>feces</topic><topic>Feces - microbiology</topic><topic>food animals</topic><topic>Food Microbiology</topic><topic>food pathogens</topic><topic>foodborne illness</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>human diseases</topic><topic>human food chain</topic><topic>Listeria</topic><topic>meat carcasses</topic><topic>meat inspection</topic><topic>microbiological quality</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Rabbit</topic><topic>rabbits</topic><topic>Rabbits - microbiology</topic><topic>risk assessment</topic><topic>Salmonella</topic><topic>Staphylococcus aureus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kohler, R.</creatorcontrib><creatorcontrib>Krause, G.</creatorcontrib><creatorcontrib>Beutin, L.</creatorcontrib><creatorcontrib>Stephan, R.</creatorcontrib><creatorcontrib>Zweifel, C.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kohler, R.</au><au>Krause, G.</au><au>Beutin, L.</au><au>Stephan, R.</au><au>Zweifel, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shedding of food-borne pathogens and microbiological carcass contamination in rabbits at slaughter</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2008-11-25</date><risdate>2008</risdate><volume>132</volume><issue>1</issue><spage>149</spage><epage>157</epage><pages>149-157</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>To obtain microbiological data from rabbits at slaughter, 500 fecal samples and 500 carcasses samples were examined. All samples tested negative for
Listeria and
Salmonella.
Campylobacter were detected in two fecal samples. Of the 500 fecal samples, 45.8% tested positive for
eae (intimin), 1.2% for
stx (Shiga toxin), and 1.8% for both
eae and
stx. By colony hybridization, 56
eae positive
Escherichia coli strains were isolated. Among them, 27 strains (48.2%) were of the serotypes O178:H7 and O153:H7, whereas 15 strains (26.8%) belonged to a serogroup that has not yet been described (O(CB10681):H7). All strains possessed intimin β1 and the translocated intimin receptor (
tir) capable of being tyrosine phosphorylated. None of the strains harbored the genes for Shiga toxins, EAST1 (
astA), bundlin (
bfpA), or the EAF plasmid. Slaughter rabbits therefore constitute a reservoir for certain atypical enteropathogenic
Escherichia coli. On rabbit carcasses, average total bacterial counts accounted for 3.3
log
CFU
cm
−2.
Enterobacteriaceae and coagulase positive staphylococci (CPS) were detected on 118 (23.6%) and 153 (30.6%) carcasses, respectively.
Enterobacteriaceae and CPS counts of positive samples were mainly <1.5
log
CFU
cm
−2. Among 153 selected CPS isolates, 98.7% were identified as
Staphylococcus aureus. None of the 151 isolated strains harbored the gene for methicillin resistance (
mecA). Genes for staphylococcal enterotoxins (SE) were detected in 102 strains. The combinations of
seg and
sei (53 strains) and
sed,
seg,
sei, and
sej (27 strains) dominated.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18514438</pmid><doi>10.1016/j.vetmic.2008.04.020</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Veterinary microbiology, 2008-11, Vol.132 (1), p.149-157 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19686700 |
| source | ScienceDirect Freedom Collection |
| subjects | Abattoirs animal pathogenic bacteria animal production Animals Bacteria - classification Bacteria - isolation & purification bacterial contamination bacterial toxins Bacteriology Biological and medical sciences Campylobacter Carcass contamination disease reservoirs Enterobacteriaceae Enteropathogenic Escherichia coli epidemiological studies Escherichia coli Fecal shedding feces Feces - microbiology food animals Food Microbiology food pathogens foodborne illness Fundamental and applied biological sciences. Psychology human diseases human food chain Listeria meat carcasses meat inspection microbiological quality Microbiology Miscellaneous Rabbit rabbits Rabbits - microbiology risk assessment Salmonella Staphylococcus aureus |
| title | Shedding of food-borne pathogens and microbiological carcass contamination in rabbits at slaughter |
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