Combinatorial selection of a single stranded DNA thioaptamer targeting TGF- beta 1 protein

A phosphorothioate single-stranded DNA aptamer (thioaptamer) targeting transforming growth factor- beta 1 (TGF- beta 1) was isolated by in-vitro combinatorial selection. The aptamer selection procedure was designed to modify the backbone of single-stranded DNA aptamers, where 5' of both A and C...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry 2008-03, Vol.18 (6), p.1835-1839
Main Authors: Kang, Jonghoon, Lee, Myung Soog, Copland, John A, Luxon, Bruce A, Gorenstein, David G
Format: Article
Language:English
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Summary:A phosphorothioate single-stranded DNA aptamer (thioaptamer) targeting transforming growth factor- beta 1 (TGF- beta 1) was isolated by in-vitro combinatorial selection. The aptamer selection procedure was designed to modify the backbone of single-stranded DNA aptamers, where 5' of both A and C are phosphorothioates, since this provides enhanced nuclease resistance as well as higher affinity than that of a phosphate counterpart. The thioaptamer selected from a combinatorial library (5 x 10 super(14) sequences) binds to TGF- beta 1 protein with an affinity of 90 nM. In this report, sequence, predicted secondary structure, and binding affinity of the selected thioaptamer (T18_1_3) are presented.
ISSN:0968-0896
1464-3391
DOI:10.1016/j.bmcl.2008.02.023